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Blood, 15 January 2006, Vol. 107, No. 2, pp. 520-527. Prepublished online as a Blood First Edition Paper on September 20, 2005; DOI 10.1182/blood-2005-04-1385. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-04-1385v1 107/2/520    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zheng, J. Articles by Nakano, T. Search for Related Content PubMed PubMed Citation Articles by Zheng, J. Articles by Nakano, T. Related Collections Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Differential effects of GATA-1 on proliferation and differentiation of erythroid lineage cells Jie Zheng, Kenji Kitajima, Eiko Sakai, Tohru Kimura, Naoko Minegishi, Masayuki Yamamoto, and Toru Nakano From the Department of Pathology, Medical School and Frontier Biosciences, Osaka University, Japan; Biomedical Engineering Research Organization, Tohoku University, Sendai, Japan; and the Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Japan. The zinc finger transcription factor GATA-1 is essential for both primitive (embryonic) and definitive (adult) erythropoiesis. To define the roles of GATA-1 in the production and differentiation of primitive and definitive erythrocytes, we established GATA-1-null embryonic stem cell lines in which GATA-1 was able to be conditionally expressed by using the tetracycline conditional gene expression system. The cells were subjected to hematopoietic differentiation by coculturing on OP9 stroma cells. We expressed GATA-1 in the course of primitive and definitive erythropoiesis and analyzed the ability of GATA-1 to rescue the defective erythropoiesis caused by the GATA-1 null mutation. Our results show that GATA-1 functions in the proliferation and maturation of erythrocytes in a distinctive manner. The early-stage expression of GATA-1 during both primitive and definitive erythropoiesis was sufficient to promote the proliferation of red blood cells. In contrast, the late-stage expression of GATA-1 was indispensable to the terminal differentiation of primitive and definitive erythrocytes. Thus, GATA-1 affects the proliferation and differentiation of erythrocytes by different mechanisms. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Gutierrez, S. Tsukamoto, M. Suzuki, H. Yamamoto-Mukai, M. Yamamoto, S. Philipsen, and K. Ohneda Ablation of Gata1 in adult mice results in aplastic crisis, revealing its essential role in steady-state and stress erythropoiesis Blood, April 15, 2008; 111(8): 4375 - 4385. [Abstract] [Full Text] [PDF] D. Sugiyama, M. Tanaka, K. Kitajima, J. Zheng, H. Yen, T. Murotani, A. Yamatodani, and T. Nakano Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation Blood, February 15, 2008; 111(4): 1924 - 1932. [Abstract] [Full Text] [PDF] C. Naujokat and T. Saric Concise Review: Role and Function of the Ubiquitin-Proteasome System in Mammalian Stem and Progenitor Cells Stem Cells, October 1, 2007; 25(10): 2408 - 2418. [Abstract] [Full Text] [PDF] K. Kitajima, J. Zheng, H. Yen, D. Sugiyama, and T. Nakano Multipotential differentiation ability of GATA-1-null erythroid-committed cells. Genes & Dev., March 15, 2006; 20(6): 654 - 659. [Abstract] [Full Text] [PDF] A. L. Olsen, D. L. Stachura, and M. J. Weiss Designer blood: creating hematopoietic lineages from embryonic stem cells Blood, February 15, 2006; 107(4): 1265 - 1275. [Abstract] [Full Text] [PDF]

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