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Blood, 1 February 2006, Vol. 107, No. 3, pp. 1141-1148. Prepublished online as a Blood First Edition Paper on October 4, 2005; DOI 10.1182/blood-2005-04-1722. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-04-1722v1 107/3/1141    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lenze, D. Articles by Hummel, M. Search for Related Content PubMed PubMed Citation Articles by Lenze, D. Articles by Hummel, M. Related Collections Immunobiology Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Influence of antigen on the development of MALT lymphoma Dido Lenze, Erika Berg, Rudolf Volkmer-Engert, Armin A. Weiser, Axel Greiner, Constanze Knörr-Wittmann, Ioannis Anagnostopoulos, Harald Stein, and Michael Hummel From the Institute of Pathology, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Germany; Institute of Medical Immunology, Campus Mitte, Charité-Universitätsmedizin Berlin, Germany; and the Institute of Pathology, University of Würzburg, Germany. Mucosa-associated lymphoid tissue (MALT) B-cell lymphomas develop in the context of autoimmune or chronic inflammations like Helicobacter pylori–induced gastritis. Remission of most gastric MALT lymphomas after eradication of H pylori links tumor cell proliferation to antigen-induced inflammation and the need for antigenic contact. Furthermore, the tumor cells correspond to antigen-activated memory B cells. To investigate the reactivity of the tumor immunoglobulins we employed in vitro–generated antibodies identical to those produced by MALT lymphoma cells. The immunoglobulin rearrangements of 7 MALT lymphomas were amplified, cloned, and expressed as single-chain fragment variable (scFv) antibodies. Antigen specificity of these 7 scFvs was analyzed by immunohistochemical staining of various normal, reactive, and malignant human tissues. Also, an expression library comprising approximately 30 000 proteins from human fetal brains (protein filter) and a peptide library were screened. One scFv stained a subpopulation of tonsillar plasma cells in immunohistochemical studies. On protein filters this scFv recognized the plasma cell–related protein Ufc1. Peptide library screening identified 9 peptides as binding partners of an additional scFv. The majority of MALT lymphoma immunoglobulins studied, however, showed no reactivity against antigens, indicating that the tumor immunoglobulins do not play a significant role in stimulation and proliferation of the MALT lymphoma tumor cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: U. Hershberg, M. Uduman, M. J. Shlomchik, and S. H. Kleinstein Improved methods for detecting selection by mutation analysis of Ig V region sequences Int. Immunol., May 1, 2008; 20(5): 683 - 694. [Abstract] [Full Text] [PDF]

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