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Blood, 1 February 2006, Vol. 107, No. 3, pp. 1214-1216. Prepublished online as a Blood First Edition Paper on September 29, 2005; DOI 10.1182/blood-2005-08-3400. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-08-3400v1 107/3/1214    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liersch, R. Articles by Detmar, M. Search for Related Content PubMed PubMed Citation Articles by Liersch, R. Articles by Detmar, M. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Stem Cells in Hematology Brief Reports Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  STEM CELLS IN HEMATOLOGY Brief report Induction of lymphatic endothelial cell differentiation in embryoid bodies Ruediger Liersch, Filip Nay, Lingge Lu, and Michael Detmar From the Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland; and the Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA. The molecular mechanisms that regulate the formation of the lymphatic vascular system remain poorly characterized. Whereas studies in embryonic stem (ES) cells have provided major new insights into the mechanisms of blood vessel formation, the development of lymphatic endothelium has not been previously observed. We established embryoid bodies (EBs) from murine ES cells in the presence or absence of lymphangiogenic growth factors. We found that lymphatic endothelial cells develop at day 18 after EB formation. These cells express CD31 and the lymphatic lineage markers Prox-1 and Lyve-1, but not the vascular marker MECA-32, and they frequently sprout from preexisting blood vessels. Lymphatic vessel formation was potently promoted by VEGF-A and VEGF-C but not by bFGF. Our results reveal, for the first time, that ES cells can differentiate into lymphatic endothelial cells, and they identify the EB assay as a powerful new tool to dissect the molecular mechanisms that control lymphatic vessel formation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Lymphangiogenesis in vitro Georg Breier Blood 2006 107: 853-854. [Full Text] [PDF] This article has been cited by other articles: M. Oka, C. Iwata, H. I. Suzuki, K. Kiyono, Y. Morishita, T. Watabe, A. Komuro, M. R. Kano, and K. Miyazono Inhibition of endogenous TGF-{beta} signaling enhances lymphangiogenesis Blood, May 1, 2008; 111(9): 4571 - 4579. [Abstract] [Full Text] [PDF] K. Kajiya, S. Hirakawa, and M. Detmar Vascular Endothelial Growth Factor-A Mediates Ultraviolet B-Induced Impairment of Lymphatic Vessel Function Am. J. Pathol., October 1, 2006; 169(4): 1496 - 1503. [Abstract] [Full Text] [PDF] J. Kreuger, I. Nilsson, D. Kerjaschki, T. Petrova, K. Alitalo, and L. Claesson-Welsh Early Lymph Vessel Development From Embryonic Stem Cells Arterioscler. Thromb. Vasc. Biol., May 1, 2006; 26(5): 1073 - 1078. [Abstract] [Full Text] [PDF]

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