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Blood, 1 February 2006, Vol. 107, No. 3, pp. 907-915. Prepublished online as a Blood First Edition Paper on October 4, 2005; DOI 10.1182/blood-2005-06-2516. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-06-2516v1 107/3/907    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhao, W. Articles by Ghaffari, S. Search for Related Content PubMed PubMed Citation Articles by Zhao, W. Articles by Ghaffari, S. Related Collections Hematopoiesis and Stem Cells Signal Transduction Free Research Articles Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Erythropoietin stimulates phosphorylation and activation of GATA-1 via the PI3-kinase/AKT signaling pathway Wei Zhao, Claire Kitidis, Mark D. Fleming, Harvey F. Lodish, and Saghi Ghaffari From the Department of Gene and Cell Medicine, Division of Hematology Oncology, Mount Sinai School of Medicine, New York, NY; the Department of Medicine, Division of Hematology Oncology, Mount Sinai School of Medicine, New York, NY; the Whitehead Institute for Biomedical Research, Cambridge MA; the Department of Pathology, Children's Hospital Harvard Medical School, Boston, MA; and the Department of Biology, Massachusetts Institute of Technology, Cambridge, MA. Erythropoietin (Epo) stimulation of its receptor's downstream signaling pathways and optimum function of GATA-1 transcription factor are both essential for normal erythroid cell development. Epo-receptor (EpoR) signaling and GATA-1 regulate proliferation, survival, differentiation, and maturation of erythroid cells. Whether any signal that is generated by EpoR targets GATA-1 or affects GATA-1 transcriptional activity is not known. Here, we demonstrate that stimulation of EpoR results in phosphorylation of GATA-1 at serine 310 (S310) in primary fetal liver erythroid progenitors and in cultured erythroid cells. We show that phosphorylation of GATA-1 is important for Epo-induced maturation of fetal liver erythroid progenitor cells. The PI3-kinase/AKT signaling pathway is identified as a mediator of Epo-induced phosphorylation of GATA-1. AKT serine threonine kinase phosphorylates GATA-1S310 in vitro and in erythroid cells and enhances GATA-1 transcriptional activity. These data demonstrate that EpoR signaling phosphorylates GATA-1 and modulates its activity via the PI3-kinase/AKT signaling pathway. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: GATA-1 caught in the AKT Gerd A. Blobel Blood 2006 107: 851a-852. [Full Text] [PDF] This article has been cited by other articles: W.-H. Leung and S. Bolland The Inositol 5'-Phosphatase SHIP-2 Negatively Regulates IgE-Induced Mast Cell Degranulation and Cytokine Production J. Immunol., July 1, 2007; 179(1): 95 - 102. [Abstract] [Full Text] [PDF] T.-l. Gu, T. Mercher, J. W. Tyner, V. L. Goss, D. K. Walters, M. G. Cornejo, C. Reeves, L. Popova, K. Lee, M. C. Heinrich, et al. A novel fusion of RBM6 to CSF1R in acute megakaryoblastic leukemia Blood, July 1, 2007; 110(1): 323 - 333. [Abstract] [Full Text] [PDF] H. K. Bone and M. J. Welham Phosphoinositide 3-kinase signalling regulates early development and developmental haemopoiesis J. Cell Sci., May 15, 2007; 120(10): 1752 - 1762. [Abstract] [Full Text] [PDF] P. Rimmele, O. Kosmider, P. Mayeux, F. Moreau-Gachelin, and C. Guillouf Spi-1/PU.1 participates in erythroleukemogenesis by inhibiting apoptosis in cooperation with Epo signaling and by blocking erythroid differentiation Blood, April 1, 2007; 109(7): 3007 - 3014. [Abstract] [Full Text] [PDF] L. O. Andrieux, A. Fautrel, A. Bessard, A. Guillouzo, G. Baffet, and S. Langouet GATA-1 Is Essential in EGF-Mediated Induction of Nucleotide Excision Repair Activity and ERCC1 Expression through ERK2 in Human Hepatoma Cells Cancer Res., March 1, 2007; 67(5): 2114 - 2123. [Abstract] [Full Text] [PDF] H. M. Rooke and S. H. Orkin Phosphorylation of Gata1 at serine residues 72, 142, and 310 is not essential for hematopoiesis in vivo Blood, May 1, 2006; 107(9): 3527 - 3530. [Abstract] [Full Text] [PDF] S. Ghaffari, C. Kitidis, W. Zhao, D. Marinkovic, M. D. Fleming, B. Luo, J. Marszalek, and H. F. Lodish AKT induces erythroid-cell maturation of JAK2-deficient fetal liver progenitor cells and is required for Epo regulation of erythroid-cell differentiation Blood, March 1, 2006; 107(5): 1888 - 1891. [Abstract] [Full Text] [PDF]

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