|
|
Blood, 15 February 2006, Vol. 107, No. 4, pp. 1357-1365.
Prepublished online as a Blood First Edition Paper on October 18, 2005; DOI 10.1182/blood-2005-05-1916.
 Previous Article | Table of Contents | Next Article 
HEMATOPOIESIS
Developmental regulation of yolk sac hematopoiesis by Krüppel-like factor 6
Nobuyuki Matsumoto,
Atsushi Kubo,
Huixian Liu,
Kuniharu Akita,
Friedrich Laub,
Francesco Ramirez,
Gordon Keller, and
Scott L. Friedman
From the Division of Liver Diseases, the Department of Medicine, and the Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY; the Laboratory of Genetics and Organogenesis, Research Division of the Hospital for Special Surgery, Department of Physiology and Biophysics, Weill Medical College, Cornell University, New York, NY; and Centro Ingegneria Genetica (CEINGE)-Biotecnologie Avanzate, Naples, Italy.
Krüppel-like factor 6 (KLF6) is a member of a growing family of transcription factors that share a common 3 C2H2 zinc finger DNA binding domain and have broad activity in regulating proliferation and development. We have previously established that Klf6 is expressed in neuronal tissue, hindgut, heart, lung, kidney, and limb buds during midgestation. To explore the potential role of Klf6 in mouse development, we analyzed Klf6-/- mice and found that the homozygous mutation is embryonic lethal by embryonic day (E) 12.5 and associated with markedly reduced hematopoiesis and poorly organized yolk sac vascularization. Additionally, mRNA levels of Scl and Gata1 were reduced by approximately 80% in Klf6-/- yolk sacs. To further analyze this phenotype, we generated Klf6-/- embryonic stem (ES) cells by homologous recombination, and compared their capacity to differentiate into the hematopoietic lineage with that of either Klf6+/- or Klf6+/+ ES cells. Consistent with the phenotype in the early embryo, Klf6-/- ES cells displayed significant hematopoietic defects following differentiation into EBs. Prolongation of epiblast-like cells and delays in mesoderm induction were also observed in the Klf6-/- EBs, associated with delayed expression of Brachyury, Klf1, and Gata1. Forced expression of KLF6 using a tet-inducible system enhanced the hematopoietic potential of wild-type EBs. Collectively, these findings implicate Klf6 in ES-cell differentiation and hematopoiesis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
N. C. Dubois, C. Adolphe, A. Ehninger, R. A. Wang, E. J. Robertson, and A. Trumpp
Placental rescue reveals a sole requirement for c-Myc in embryonic erythroblast survival and hematopoietic stem cell function
Development,
July 15, 2008;
135(14):
2455 - 2465.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Alder, R. W. Georgantas III, R. L. Hildreth, I. M. Kaplan, S. Morisot, X. Yu, M. McDevitt, and C. I. Civin
Kruppel-Like Factor 4 Is Essential for Inflammatory Monocyte Differentiation In Vivo
J. Immunol.,
April 15, 2008;
180(8):
5645 - 5652.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Bentov, G. Narla, H. Schayek, K. Akita, S. R. Plymate, D. LeRoith, S. L. Friedman, and H. Werner
Insulin-Like Growth Factor-I Regulates Kruppel-Like Factor-6 Gene Expression in a p53-Dependent Manner
Endocrinology,
April 1, 2008;
149(4):
1890 - 1897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Basu, T. K. Lung, W. Lemsaddek, T. G. Sargent, D. C. Williams Jr, M. Basu, L. C. Redmond, J. B Lingrel, J. L. Haar, and J. A. Lloyd
EKLF and KLF2 have compensatory roles in embryonic {beta}-globin gene expression and primitive erythropoiesis
Blood,
November 1, 2007;
110(9):
3417 - 3425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kobayashi and M. Yamamoto
Regulation of GATA1 Gene Expression
J. Biochem.,
July 1, 2007;
142(1):
1 - 10.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. W. Funnell, C. A. Maloney, L. J. Thompson, J. Keys, M. Tallack, A. C. Perkins, and M. Crossley
Erythroid Kruppel-Like Factor Directly Activates the Basic Kruppel-Like Factor Gene in Erythroid Cells
Mol. Cell. Biol.,
April 1, 2007;
27(7):
2777 - 2790.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
