SEARCH:   Advanced

Blood, 1 March 2006, Vol. 107, No. 5, pp. 2146-2152. Prepublished online as a Blood First Edition Paper on October 25, 2005; DOI 10.1182/blood-2005-08-3139. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-08-3139v1 107/5/2146    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Giebel, B. Articles by Punzel, M. Search for Related Content PubMed PubMed Citation Articles by Giebel, B. Articles by Punzel, M. Related Collections Hematopoiesis and Stem Cells Stem Cells in Hematology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  STEM CELLS IN HEMATOLOGY Primitive human hematopoietic cells give rise to differentially specified daughter cells upon their initial cell division Bernd Giebel, Tao Zhang, Julia Beckmann, Jan Spanholtz, Peter Wernet, Anthony D. Ho, and Michael Punzel From the Institute for Transplantation Diagnostics and Cellular Therapeutics, Heinrich-Heine-University Düsseldorf, Germany; Department of Medicine V, Ruprecht-Karls University of Heidelberg, Germany; and Cancer Center Union Hospital, Huazhong University of Science and Technology Wuhan, People's Republic of China. It is often predicted that stem cells divide asymmetrically, creating a daughter cell that maintains the stem-cell capacity, and 1 daughter cell committed to differentiation. While asymmetric stem-cell divisions have been proven to occur in model organisms (eg, in Drosophila), it remains illusive whether primitive hematopoietic cells in mammals actually can divide asymmetrically. In our experiments we have challenged this question and analyzed the developmental capacity of separated offspring of primitive human hematopoietic cells at a single-cell level. We show for the first time that the vast majority of the most primitive, in vitro–detectable human hematopoietic cells give rise to daughter cells adopting different cell fates; 1 inheriting the developmental capacity of the mother cell, and 1 becoming more specified. In contrast, approximately half of the committed progenitor cells studied gave rise to daughter cells, both of which adopted the cell fate of their mother. Although our data are compatible with the model of asymmetric cell division, other mechanisms of cell fate specification are discussed. In addition, we describe a novel human hematopoietic progenitor cell that has the capacity to form natural killer (NK) cells as well as macrophages, but not cells of other myeloid lineages. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. A. Martinez-Agosto, H. K.A. Mikkola, V. Hartenstein, and U. Banerjee The hematopoietic stem cell and its niche: a comparative view Genes & Dev., December 1, 2007; 21(23): 3044 - 3060. [Abstract] [Full Text] [PDF] J. Beckmann, S. Scheitza, P. Wernet, J. C. Fischer, and B. Giebel Asymmetric cell division within the human hematopoietic stem and progenitor cell compartment: identification of asymmetrically segregating proteins Blood, June 15, 2007; 109(12): 5494 - 5501. [Abstract] [Full Text] [PDF] S. Gottschling, R. Saffrich, A. Seckinger, U. Krause, K. Horsch, K. Miesala, and A. D. Ho Human Mesenchymal Stromal Cells Regulate Initial Self-Renewing Divisions of Hematopoietic Progenitor Cells by a {beta}1-Integrin-Dependent Mechanism Stem Cells, March 1, 2007; 25(3): 798 - 806. [Abstract] [Full Text] [PDF] A. Noer, A. L. Sorensen, A. C. Boquest, and P. Collas Stable CpG Hypomethylation of Adipogenic Promoters in Freshly Isolated, Cultured, and Differentiated Mesenchymal Stem Cells from Adipose Tissue Mol. Biol. Cell, August 1, 2006; 17(8): 3543 - 3556. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020