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Blood, 15 March 2006, Vol. 107, No. 6, pp. 2243-2251. Prepublished online as a Blood First Edition Paper on November 15, 2005; DOI 10.1182/blood-2005-02-0581. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-02-0581v1 107/6/2243    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Foudi, A. Articles by Louache, F. Search for Related Content PubMed PubMed Citation Articles by Foudi, A. Articles by Louache, F. Related Collections Hematopoiesis and Stem Cells Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES, CYTOKINES, AND INTERLEUKINS Reduced retention of radioprotective hematopoietic cells within the bone marrow microenvironment in CXCR4–/– chimeric mice Adlen Foudi, Peggy Jarrier, Yanyan Zhang, Monika Wittner, Jean-Francois Geay, Yann Lecluse, Takashi Nagasawa, William Vainchenker, and Fawzia Louache From the National Institute for Health and Medical Research (INSERM) U362, IFR54, Institut Gustave Roussy, Villejuif, France; and Department of Medical Systems Control, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku Kyoto, Japan. The physiologic role of CXCR4 on hematopoietic stem/progenitor cells (HSPCs) is not fully understood. Here, we show that radioprotection of lethally irradiated mice by embryonic day 14.5 (E14.5) CXCR4–/– fetal liver (FL) cells was markedly impaired when compared with CXCR4+/+ counterparts, but this defect was rescued when hosts were engrafted with high cell numbers. This quantitative defect contrasted with a similar content in hematopoietic colony-forming cells (CFCs), splenic colony-forming units (CFUs-S), and Lin– Sca-1+ c-kit+ cells in E14.5 CXCR4–/– and CXCR4+/+ livers. In addition, the homing of HSPCs in the bone marrow was not altered as detected with a CFSE-staining assay. In contrast, a 30-fold increase in CFCs was seen in the circulation of mice stably reconstituted with CXCR4–/– FL cells and this increment was already observed before hematopoiesis had reached a steady-state level. Together, the data strongly suggest that impaired retention may, at least in short-term hematopoietic reconstitution, lead to a diminution in the number of available progenitors required for radioprotection. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Papayannopoulou and D. T. Scadden Stem-cell ecology and stem cells in motion Blood, April 15, 2008; 111(8): 3923 - 3930. [Abstract] [Full Text] [PDF] Y. Nie, Y.-C. Han, and Y.-R. Zou CXCR4 is required for the quiescence of primitive hematopoietic cells J. Exp. Med., April 14, 2008; 205(4): 777 - 783. [Abstract] [Full Text] [PDF] H.-D. Chae, K. E. Lee, D. A. Williams, and Y. Gu Cross-talk between RhoH and Rac1 in regulation of actin cytoskeleton and chemotaxis of hematopoietic progenitor cells Blood, March 1, 2008; 111(5): 2597 - 2605. [Abstract] [Full Text] [PDF] G. V. Priestley, T. Ulyanova, and T. Papayannopoulou Sustained alterations in biodistribution of stem/progenitor cells in Tie2Cre+{alpha}4f/f mice are hematopoietic cell autonomous Blood, January 1, 2007; 109(1): 109 - 111. [Abstract] [Full Text] [PDF] H. Bonig, K.-H. Chang, B. Nakamoto, and T. Papayannopoulou The p67 laminin receptor identifies human erythroid progenitor and precursor cells and is functionally important for their bone marrow lodgment Blood, August 15, 2006; 108(4): 1230 - 1233. [Abstract] [Full Text] [PDF]

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