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Blood, 15 March 2006, Vol. 107, No. 6, pp. 2252-2261. Prepublished online as a Blood First Edition Paper on November 29, 2005; DOI 10.1182/blood-2005-05-2011. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-05-2011v1 107/6/2252    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Amin, M. A. Articles by Koch, A. E. Search for Related Content PubMed PubMed Citation Articles by Amin, M. A. Articles by Koch, A. E. Related Collections Immunobiology Cell Adhesion and Motility Signal Transduction Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES, CYTOKINES, AND INTERLEUKINS Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFB M. Asif Amin, Christian S. Haas, Kui Zhu, Pamela J. Mansfield, Michael J. Kim, Nicholas P. Lackowski, and Alisa E. Koch From the Department of Medicine, University of Michigan Medical School, Ann Arbor; the Department of Medicine, Northwestern University Medical School, Chicago, IL; the Veterans Administration Chicago Health Care Medical Center, Lakeside Division, Chicago, IL; and the Veterans Administration, Ann Arbor, MI. Cell adhesion molecules are critical in monocyte (MN) recruitment in immune-mediated and hematologic diseases. We investigated the novel role of recombinant human migration inhibitory factor (rhMIF) in up-regulating vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) and their signaling pathways in human MNs. rhMIF-induced expression of VCAM-1 and ICAM-1 was significantly higher compared with nonstimulated MNs. rhMIF induced MN VCAM-1 and ICAM-1 expression in a concentration-dependent manner (P < .05). Antisense oligodeoxynucleotides (ODNs) and inhibitors of Src, PI3K, p38, and NFB significantly reduced rhMIF-induced MN VCAM-1 and ICAM-1 expression (P < .05). However, Erk1/2 and Jak2 were not involved. Silencing RNA directed against MIF, and inhibitors of Src, PI3K, NFB, anti–VCAM-1, and anti–ICAM-1 significantly inhibited rhMIF-induced adhesion of HL-60 cells to human dermal microvascular endothelial cells (HMVECs) or an endothelial cell line, HMEC-1, in cell adhesion assays, suggesting the functional significance of MIF-induced adhesion molecules (P < .05). rhMIF also activated MN phospho-Src, -Akt, and -NFB in a time-dependent manner. rhMIF induced VCAM-1 and ICAM-1 up-regulation in 12 hours via Src, PI3K, and NFB as shown by Western blotting and immunofluorescence. MIF and MIF-dependent signaling pathways may be a potential target for treating diseases characterized by up-regulation of cell adhesion molecules. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Carli, C. N. Metz, Y. Al-Abed, P. H. Naccache, and A. Akoum Up-Regulation of Cyclooxygenase-2 Expression and Prostaglandin E2 Production in Human Endometriotic Cells by Macrophage Migration Inhibitory Factor: Involvement of Novel Kinase Signaling Pathways Endocrinology, July 1, 2009; 150(7): 3128 - 3137. [Abstract] [Full Text] [PDF] B. J. Rabquer, Y. Hou, F. Del Galdo, G. Kenneth Haines III, M. L. Gerber, S. A. Jimenez, J. R. Seibold, and A. E. Koch The proadhesive phenotype of systemic sclerosis skin promotes myeloid cell adhesion via ICAM-1 and VCAM-1 Rheumatology, July 1, 2009; 48(7): 734 - 740. [Abstract] [Full Text] [PDF] C. N. Paiva, R. H. Arras, E. S. Magalhaes, L. S. Alves, L. P. Lessa, M. H. Silva, R. Ejzemberg, C. Canetti, and M. T. Bozza Migration inhibitory factor (MIF) released by macrophages upon recognition of immune complexes is critical to inflammation in Arthus reaction J. Leukoc. Biol., May 1, 2009; 85(5): 855 - 861. [Abstract] [Full Text] [PDF] L. M. Marsh, L. Cakarova, G. Kwapiszewska, W. von Wulffen, S. Herold, W. 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