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Blood, 1 April 2006, Vol. 107, No. 7, pp. 2602-2604. Prepublished online as a Blood First Edition Paper on December 8, 2005; DOI 10.1182/blood-2005-10-4104. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-10-4104v1 107/7/2602    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Huang, K. T. Articles by Kim-Shapiro, D. B. Search for Related Content PubMed PubMed Citation Articles by Huang, K. T. Articles by Kim-Shapiro, D. B. Related Collections Red Cells Brief Reports Related Letters in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PLENARY PAPERS Lack of allosterically controlled intramolecular transfer of nitric oxide from the heme to cysteine in the subunit of hemoglobin Kris T. Huang, Ivan Azarov, Swati Basu, Jinming Huang, and Daniel B. Kim-Shapiro From the Department of Biomedical Engineering, Wake Forest University School of Medicine; and Department of Physics and Department of Chemistry, Wake Forest University, Winston-Salem, NC. The SNO-Hb hypothesis holds that heme-bound nitric oxide (NO) present in the subunits of T-state hemoglobin (Hb) will be transferred to the -93 cysteine upon conversion to R-state Hb, thereby forming SNO-Hb. A deficiency in the ability of Hb to facilitate this intramolecular transfer has recently been purported to play a role in pulmonary hypertension and sickle cell disease. We prepared deoxygenated Hb samples with small amounts of heme-bound NO and then oxygenated the samples. Electron paramagnetic resonance (EPR) spectroscopy was used to (1) determine the concentration of iron nitrosyl Hb (Fe-NO Hb), (2) show that the NO is evenly distributed among and subunits, and (3) show that the Hb undergoes a change in its quaternary state (T to R) upon oxygenation. We did not observe a decrease in the concentration of Fe-NO Hb on oxygenation, which is inconsistent with the prediction of the SNO-Hb hypothesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letters in Blood Online: NO, SNO, and hemoglobin: lessons in complexity Andrew J. Gow and David Singel Blood 2006 108: 3224-3225. [Full Text] [PDF] Detecting physiologic fluctuations in the S-nitrosohemoglobin micropopulation: triiodide versus 3C Allan Doctor, Benjamin Gaston, and Daniel B. Kim-Shapiro Blood 2006 108: 3225-3227. [Full Text] [PDF] This article has been cited by other articles: M. T. Gladwin and D. B. Kim-Shapiro The functional nitrite reductase activity of the heme-globins Blood, October 1, 2008; 112(7): 2636 - 2647. [Abstract] [Full Text] [PDF] S. C. Rogers, A. Khalatbari, B. N. Datta, S. Ellery, V. Paul, M. P. Frenneaux, and P. E. James NO metabolite flux across the human coronary circulation Cardiovasc Res, July 15, 2007; 75(2): 434 - 441. [Abstract] [Full Text] [PDF] R. Grubina, Z. Huang, S. Shiva, M. S. Joshi, I. Azarov, S. Basu, L. A. Ringwood, A. Jiang, N. Hogg, D. B. Kim-Shapiro, et al. Concerted Nitric Oxide Formation and Release from the Simultaneous Reactions of Nitrite with Deoxy- and Oxyhemoglobin J. Biol. Chem., April 27, 2007; 282(17): 12916 - 12927. [Abstract] [Full Text] [PDF] A. J. Gow and D. Singel NO, SNO, and hemoglobin: lessons in complexity. Blood, November 1, 2006; 108(9): 3224 - 3225. [Full Text] [PDF] A. Doctor, B. Gaston, and D. B. Kim-Shapiro Detecting physiologic fluctuations in the S-nitrosohemoglobin micropopulation: triiodide versus 3C. Blood, November 1, 2006; 108(9): 3225 - 3227. [Full Text] [PDF] M. T. Gladwin, N. J. H. Raat, S. Shiva, C. Dezfulian, N. Hogg, D. B. Kim-Shapiro, and R. P. Patel Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2026 - H2035. [Abstract] [Full Text] [PDF] B. W. Allen and C. A. Piantadosi How do red blood cells cause hypoxic vasodilation? The SNO-hemoglobin paradigm Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1507 - H1512. [Abstract] [Full Text] [PDF]

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