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Blood, 15 April 2006, Vol. 107, No. 8, pp. 3389-3396. Prepublished online as a Blood First Edition Paper on December 20, 2005; DOI 10.1182/blood-2005-08-3431. This Article Full Text Full Text (PDF) Supplemental Table and Figure All Versions of this Article: 2005-08-3431v1 107/8/3389    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Faber, P. Articles by Spyridonidis, A. Search for Related Content PubMed PubMed Citation Articles by Faber, P. Articles by Spyridonidis, A. Related Collections Transplantation Clinical Trials and Observations Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Frequent genomic alterations in epithelium measured by microsatellite instability following allogeneic hematopoietic cell transplantation in humans Philipp Faber, Paul Fisch, Miguel Waterhouse, Annette Schmitt-Gräff, Hartmut Bertz, Jürgen Finke, and Alexandros Spyridonidis From the Departments of Hematology/Oncology and of Pathology, Albert Ludwigs University, Freiburg, Germany. Although typically found in cancers, frameshift mutations in microsatellites have also been detected in chronically inflamed tissues. Allogeneic hematopoietic cell transplantation (HCT) may potentially produce chronic tissue stress through graft-versus-host reactions. We examined non-neoplastic epithelial tissues (colon, buccal) obtained 1 to 5061 days after human allogeneic HCT for the presence of genomic alterations at 3 tetranucleotide and 3 mononucleotide microsatellite loci. Novel bands indicative of microsatellite instability (MSI) at tetranucleotide repeats were detected in laser-microdissected colonic crypts and in buccal smears of 75% and 42% of patients who received an allograft, respectively. In contrast, no MSI was found in similar tissues from control subjects and from patients after intensive chemotherapy or in buccal cells from patients after autologous HCT. The MSI found in colon, which was often affected by graft-versus-host disease, was not due to loss of expression or nitrosylation of DNA repair proteins. MSI in clinically intact oral mucosa was more frequently found at later time points after HCT. MSI was also found in 3 posttransplant squamous cell cancers examined. Our data show that genomic alterations in epithelium regularly occur after allogeneic HCT and may be implicated in the evolution of posttransplantation diseases, including secondary cancer. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Predicting secondary malignancies after allogeneic transplantation? Gérard Socié Blood 2006 107: 3022-3023. [Full Text] [PDF] This article has been cited by other articles: A. Janin, H. Murata, C. Leboeuf, J.-M. Cayuela, E. Gluckman, L. Legres, A. Desveaux, M. Varna, P. Ratajczak, J. Soulier, et al. Donor-derived oral squamous cell carcinoma after allogeneic bone marrow transplantation Blood, February 19, 2009; 113(8): 1834 - 1840. [Abstract] [Full Text] [PDF] M. Themeli, L. Petrikkos, M. Waterhouse, H. Bertz, E. Lagadinou, N. Zoumbos, J. Finke, and A. Spyridonidis Alloantigeneic Reactions after Human Hematopoietic Cell Transplantation Induce Genomic Alterations in Epithelium: A Confirmation in Vivo and in Vitro Study and Implications to Secondary Neoplasia Blood (ASH Annual Meeting Abstracts), November 16, 2008; 112(11): 452 - 452. [Abstract] A. Spyridonidis, P. Faber, L. Petrikkos, and J. Finke Alloanatigenic Reactions after Hematopoietic Cell Transplantation Induce Genomic Alterations in Epithelial Cells as Shown in Human Studies and in an In Vitro Model. Blood (ASH Annual Meeting Abstracts), November 16, 2006; 108(11): 3213 - 3213. [Abstract] [PDF]

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