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Blood, 1 July 2006, Vol. 108, No. 1, pp. 262-269. Prepublished online as a Blood First Edition Paper on March 7, 2006; DOI 10.1182/blood-2005-07-2878. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-07-2878v1 108/1/262    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Maeda, K. Articles by Ogawa, H. Search for Related Content PubMed PubMed Citation Articles by Maeda, K. Articles by Ogawa, H. Related Collections Hematopoiesis and Stem Cells Immunobiology Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY FOG-1 represses GATA-1-dependent FcRI -chain transcription: transcriptional mechanism of mast-cell-specific gene expression in mice Keiko Maeda, Chiharu Nishiyama, Tomoko Tokura, Hiroyasu Nakano, Shunsuke Kanada, Makoto Nishiyama, Ko Okumura, and Hideoki Ogawa From the Atopy (Allergy) Research Center and Department of Immunology, Juntendo University School of Medicine, Tokyo; and Biotechnology Research Center, The University of Tokyo, Japan. Cell-type-specific transcription of mouse high-affinity IgE receptor (FcRI) -chain is positively regulated by the transcription factor GATA-1. Although GATA-1 is expressed in erythroid cells, megakaryocytes, and mast cells, the expression of mouse FcRI -chain is restricted to mast cells. In the present study, we characterized the role of GATA-associated cofactor FOG-1 in the regulation of the FcRI -chain promoter. The expression levels of FOG-1, GATA-1, and -chain in each hematopoietic cell line were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. FOG-1 expression was higher in the -chain-negative hematopoietic progenitor cell line Ba/F3 than in the -chain-positive mast cell line PT18. By contrast, GATA-1 expression was similar when comparing the 2 cell lines. A transient reporter assay demonstrated that the -chain promoter functioned in PT18 but not in Ba/F3 and that the transcription activity of the -chain promoter in PT18 was markedly suppressed by overexpression of FOG-1. Although the activity of the -chain promoter, which was upregulated by coexpression of GATA-1, was significantly suppressed by coexpression of FOG-1 in the simian kidney CV-1 cells (-chain-, GATA-1-, and FOG-1-), the transactivation of the -chain promoter by the GATA-1 mutant V205G, which cannot bind FOG-1, was not affected by coexpression of FOG-1. Further, overexpression of FOG-1 in PT18 resulted in decreases in cell surface expression of FcRI and -chain transcription. Finally, suppression of FOG-1 expression using an siRNA approach resulted in increased -chain promoter activity in Ba/F3. These results suggest that FOG-1 expression level regulates the GATA-1-dependent FcRI -chain promoter. (Blood. 2006;108:262-269) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. K. Miller, H. Qadota, T. J. Stark, K. B. Mercer, T. S. Wortham, A. Anyanful, and G. M. 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