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Blood, 1 December 2006, Vol. 108, No. 12, pp. 3843-3850. Prepublished online as a Blood First Edition Paper on July 25, 2006; DOI 10.1182/blood-2006-05-021535. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-05-021535v1 108/12/3843    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Montagna, D. Articles by Casorati, G. Search for Related Content PubMed PubMed Citation Articles by Montagna, D. Articles by Casorati, G. Related Collections Immunobiology Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Emergence of antitumor cytolytic T cells is associated with maintenance of hematologic remission in children with acute myeloid leukemia Daniela Montagna, Rita Maccario, Franco Locatelli, Enrica Montini, Sara Pagani, Federico Bonetti, Liane Daudt, Ilaria Turin, Daniela Lisini, Claudio Garavaglia, Paolo Dellabona, and Giulia Casorati From the Department of Pediatrics, Laboratory of Immunology, University of Pavia, Italy; Pediatric Hematology Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Matteo, Pavia, Italy; and Experimental Immunology Unit, Cancer Immunotherapy and Gene Therapy Program, Department of Oncology, Department of Biological and Technological Research (DIBIT), Hospital San Raffaele Scientific Institute, Milan, Italy. Although the graft-versus-leukemia effect of allogeneic bone marrow transplantation (BMT) is of paramount importance in the maintenance of disease remission, the role played by the autologous T-cell response in antitumor immune surveillance is less defined. We evaluated the emergence of antileukemia cytotoxic T-lymphocyte precursors (CTLp's) and the correlation of this phenomenon with maintenance of hematologic remission in 16 children with acute myeloid leukemia (AML), treated with either chemotherapy alone (5 patients) or with autologous BMT (A-BMT, 11 patients). Antileukemia CTLp's were detectable in 8 patients in remission after induction chemotherapy; none of them subsequently had a relapse. Of the 8 patients who did not show detectable CTLp frequency while in remission after induction chemotherapy, 7 subsequently experienced leukemia relapse. In patients undergoing A-BMT, molecular fingerprinting of the TCR-V repertoire, performed on antileukemia lines, demonstrated that selected antileukemia T-cell clonotypes, detectable in bone marrow before transplantation, survived ex vivo pharmacologic purging and were found in the recipient after A-BMT. These data provide evidence for an active role of autologous T cells in the maintenance of hematologic remission and also suggest that quantification of antileukemia CTLp frequency may be a useful tool to identify patients at high risk for relapse, thus potentially benefiting from an allogeneic antitumor effect. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Ghosh, W. Koestner, M. Hapke, V. Schlaphoff, F. Langer, R. Baumann, C. Koenecke, M. Cornberg, K. Welte, B. R. Blazar, et al. Donor T cells primed on leukemia lysate-pulsed recipient APCs mediate strong graft-versus-leukemia effects across MHC barriers in full chimeras Blood, April 30, 2009; 113(18): 4440 - 4448. [Abstract] [Full Text] [PDF]

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