SEARCH:   Advanced

Blood, 15 December 2006, Vol. 108, No. 13, pp. 3976-3978. Prepublished online as a Blood First Edition Paper on August 22, 2006; DOI 10.1182/blood-2006-05-024075. This Article Full Text Full Text (PDF) Supplemental Video All Versions of this Article: blood-2006-05-024075v1 108/13/3976    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Renshaw, S. A. Articles by Whyte, M. K.B. Search for Related Content PubMed PubMed Citation Articles by Renshaw, S. A. Articles by Whyte, M. K.B. Related Collections Hemostasis, Thrombosis, and Vascular Biology Phagocytes Free Research Articles Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PLENARY PAPERS A transgenic zebrafish model of neutrophilic inflammation Stephen A. Renshaw, Catherine A. Loynes, Daniel M.I. Trushell, Stone Elworthy, Philip W. Ingham, and Moira K.B. Whyte From the MRC Centre for Developmental and Biomedical Genetics, the Academic Unit of Respiratory Medicine, School of Medicine and Biomedical Sciences, and the Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom. We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced after transection of the tail of zebrafish larvae and that this inflammation subsequently resolves over a similar time course to mammalian systems. Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally induced inflammation can be inhibited by the addition of a pancaspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Fluorescent neutrophils throw the spotlight on inflammation Graham J. Lieschke Blood 2006 108: 3961-3962. [Full Text] [PDF] This article has been cited by other articles: A. Lin, J. A. Loughman, B. H. Zinselmeyer, M. J. Miller, and M. G. Caparon Streptolysin S Inhibits Neutrophil Recruitment during the Early Stages of Streptococcus pyogenes Infection Infect. Immun., November 1, 2009; 77(11): 5190 - 5201. [Abstract] [Full Text] [PDF] C. Urbinati, S. Nicoli, M. Giacca, G. David, S. Fiorentini, A. Caruso, M. Alfano, L. Cassetta, M. Presta, and M. Rusnati HIV-1 Tat and heparan sulfate proteoglycan interaction: a novel mechanism of lymphocyte adhesion and migration across the endothelium Blood, October 8, 2009; 114(15): 3335 - 3342. [Abstract] [Full Text] [PDF] I. M. Packham, C. Gray, P. R. Heath, P. G. Hellewell, P. W. Ingham, D. C. Crossman, M. Milo, and T. J. A. Chico Microarray profiling reveals CXCR4a is downregulated by blood flow in vivo and mediates collateral formation in zebrafish embryos Physiol Genomics, August 7, 2009; 38(3): 319 - 327. [Abstract] [Full Text] [PDF] M. E. Dodd, J. Hatzold, J. R. Mathias, K. B. Walters, D. A. Bennin, J. Rhodes, J. P. Kanki, A. T. Look, M. Hammerschmidt, and A. Huttenlocher The ENTH domain protein Clint1 is required for epidermal homeostasis in zebrafish Development, August 1, 2009; 136(15): 2591 - 2600. [Abstract] [Full Text] [PDF] C. Hall, M. V. Flores, A. Chien, A. Davidson, K. Crosier, and P. Crosier Transgenic zebrafish reporter lines reveal conserved Toll-like receptor signaling potential in embryonic myeloid leukocytes and adult immune cell lineages J. Leukoc. Biol., May 1, 2009; 85(5): 751 - 765. [Abstract] [Full Text] [PDF] O. W. Stockhammer, A. Zakrzewska, Z. Hegedus, H. P. Spaink, and A. H. Meijer Transcriptome Profiling and Functional Analyses of the Zebrafish Embryonic Innate Immune Response to Salmonella Infection J. Immunol., May 1, 2009; 182(9): 5641 - 5653. [Abstract] [Full Text] [PDF] L. Pase, J. E. Layton, W. P. Kloosterman, D. Carradice, P. M. Waterhouse, and G. J. Lieschke miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2 Blood, February 19, 2009; 113(8): 1794 - 1804. [Abstract] [Full Text] [PDF] F. J. Roca, I. Mulero, A. Lopez-Munoz, M. P. Sepulcre, S. A. Renshaw, J. Meseguer, and V. Mulero Evolution of the Inflammatory Response in Vertebrates: Fish TNF-{alpha} Is a Powerful Activator of Endothelial Cells but Hardly Activates Phagocytes J. Immunol., October 1, 2008; 181(7): 5071 - 5081. [Abstract] [Full Text] [PDF] A. Cvejic, C. Hall, M. Bak-Maier, M. V. Flores, P. Crosier, M. J. Redd, and P. Martin Analysis of WASp function during the wound inflammatory response - live-imaging studies in zebrafish larvae J. Cell Sci., October 1, 2008; 121(19): 3196 - 3206. [Abstract] [Full Text] [PDF] A. S. Cowburn, A. M. Condliffe, N. Farahi, C. Summers, and E. R. Chilvers Advances in Neutrophil Biology: Clinical Implications Chest, September 1, 2008; 134(3): 606 - 612. [Abstract] [Full Text] [PDF] D. Carradice and G. J. Lieschke Zebrafish in hematology: sushi or science? Blood, April 1, 2008; 111(7): 3331 - 3342. [Abstract] [Full Text] [PDF] J. Y. Bertrand, A. D. Kim, E. P. Violette, D. L. Stachura, J. L. Cisson, and D. Traver Definitive hematopoiesis initiates through a committed erythromyeloid progenitor in the zebrafish embryo Development, December 1, 2007; 134(23): 4147 - 4156. [Abstract] [Full Text] [PDF] L. K. Mathew, S. Sengupta, A. Kawakami, E. A. Andreasen, C. V. Lohr, C. A. Loynes, S. A. Renshaw, R. T. Peterson, and R. L. Tanguay Unraveling Tissue Regeneration Pathways Using Chemical Genetics J. Biol. Chem., November 30, 2007; 282(48): 35202 - 35210. [Abstract] [Full Text] [PDF] J. R. Mathias, M. E. Dodd, K. B. Walters, J. Rhodes, J. P. Kanki, A. T. Look, and A. Huttenlocher Live imaging of chronic inflammation caused by mutation of zebrafish Hai1 J. Cell Sci., October 1, 2007; 120(19): 3372 - 3383. [Abstract] [Full Text] [PDF] S. B. Brown, C. S. Tucker, C. Ford, Y. Lee, D. R. Dunbar, and J. J. Mullins Class III antiarrhythmic methanesulfonanilides inhibit leukocyte recruitment in zebrafish J. Leukoc. Biol., July 1, 2007; 82(1): 79 - 84. [Abstract] [Full Text] [PDF] StephenA. Renshaw, CatherineA. Loynes, DanielM. Trushell, PhilipW. Ingham, and MoiraK. B. Whyte The molecular controls of resolution of inflammation: what can we learn from zebrafish? Eur. Respir. Rev., December 1, 2006; 15(101): 168 - 169. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020