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Blood, 15 December 2006, Vol. 108, No. 13, pp. 4071-4077. Prepublished online as a Blood First Edition Paper on August 17, 2006; DOI 10.1182/blood-2006-04-016980. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-04-016980v1 108/13/4071    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wang, S. Articles by Yi, Q. Search for Related Content PubMed PubMed Citation Articles by Wang, S. Articles by Yi, Q. Related Collections Immunobiology Signal Transduction Immunotherapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Optimizing immunotherapy in multiple myeloma: restoring the function of patients' monocyte-derived dendritic cells by inhibiting p38 or activating MEK/ERK MAPK and neutralizing interleukin-6 in progenitor cells Siqing Wang, Sungyoul Hong, Jing Yang, Jianfei Qian, Xiang Zhang, Elizabeth Shpall, Larry W. Kwak, and Qing Yi From the Department of Lymphoma and Myeloma, the Center for Cancer Immunology Research, and the Department of Blood and Marrow Transplantation, Division of Cancer Medicine, University of Texas M. D. Anderson Cancer Center, Houston, TX. Previous studies demonstrated that circulating dendritic cells (DCs) in myeloma patients were functionally abnormal. However, the phenotype and function of patients' monocyte-derived DCs (MoDCs), which are commonly used for immunotherapy, were poorly defined. This study was undertaken to examine the quality of MoDCs from myeloma patients compared with cells from healthy donors. We found that patient-derived MoDCs are phenotypically and functionally defective. Compared with their normal counterparts, patient-derived, mature MoDCs expressed significantly lower levels of CD1a, CD40, CD80, and HLA-DR and were poor at activating alloreactive T cells, presenting recall antigen, and activating autologous antigen- and myeloma-specific T cells. These abnormalities may be attributed to elevated production of autocrine cytokines such as IL-6, activated p38 and STAT3, and inhibited MEK/ERK signaling pathways in the progenitor cells. Treatment with neutralizing IL-6–specific antibody and, more importantly, p38 inhibitor, or both, could correct these abnormalities. Treating patient-derived cells with these agents not only significantly increased cell yield but also produced MoDCs that were as functional as their normal counterparts. Thus, this study has delineated the mechanistic defects of MoDCs from myeloma patients and identified ways for restoring the function of the cells to improve the efficacy of DC-based immunotherapy in this disease. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Zhao, C. Falk, W. Osen, M. Kato, D. Schadendorf, and V. Umansky Activation of p38 Mitogen-Activated Protein Kinase Drives Dendritic Cells to Become Tolerogenic in Ret Transgenic Mice Spontaneously Developing Melanoma Clin. Cancer Res., July 1, 2009; 15(13): 4382 - 4390. [Abstract] [Full Text] [PDF] K. Vanderkerken, S. Medicherla, L. Coulton, H. De Raeve, A. Willems, M. Lawson, B. Van Camp, A. A. Protter, L. S. Higgins, E. Menu, et al. Inhibition of p38{alpha} Mitogen-Activated Protein Kinase Prevents the Development of Osteolytic Bone Disease, Reduces Tumor Burden, and Increases Survival in Murine Models of Multiple Myeloma Cancer Res., May 15, 2007; 67(10): 4572 - 4577. [Abstract] [Full Text] [PDF] F. v. Rhee Idiotype Vaccination Strategies in Myeloma: How to Overcome a Dysfunctional Immune System Clin. Cancer Res., March 1, 2007; 13(5): 1353 - 1355. [Full Text] [PDF]

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