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Blood, 15 December 2006, Vol. 108, No. 13, pp. 4214-4222. Prepublished online as a Blood First Edition Paper on August 17, 2006; DOI 10.1182/blood-2006-04-017160. This Article Full Text Full Text (PDF) Supplemental Figures, Table, and Videos All Versions of this Article: blood-2006-04-017160v1 108/13/4214    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ortolan, E. Articles by Funaro, A. Search for Related Content PubMed PubMed Citation Articles by Ortolan, E. Articles by Funaro, A. Related Collections Phagocytes Cell Adhesion and Motility Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES CD157 plays a pivotal role in neutrophil transendothelial migration Erika Ortolan, Elena V. Tibaldi, Bruna Ferranti, Luisa Lavagno, Giovanni Garbarino, Rosario Notaro, Lucio Luzzatto, Fabio Malavasi, and Ada Funaro From the Laboratory of Immunogenetics, Department of Genetics, Biology and Biochemistry, University of Torino, Torino, Italy; Research Center for Experimental Medicine (CeRMS), Torino, Italy; Department of Internal Medicine, University of Torino, Torino, Italy; National Institute for Cancer Research, Genova, Italy; and University of Genova, Genova, Italy. Paracellular diapedesis, a key step in leukocyte recruitment to the site of inflammation, occurs at endothelial junctions and is regulated by highly coordinated interactions between leukocytes and endothelium. We found that CD157, a glycosylphosphatidylinositol-anchored ectoenzyme belonging to the NADase/ADP-ribosyl cyclase family, plays a crucial role for neutrophil diapedesis, because its ligation with specific monoclonal antibodies (both on neutrophils or endothelial cells) results in altered neutrophil movement on the apical surface of endothelium and, ultimately, in loss of diapedesis. Real-time microscopy revealed that CD157 behaves as a sort of compass during the interaction between neutrophils and endothelial cells; indeed, following CD157 ligation, neutrophils appear disoriented, meandering toward junctions where they eventually stop without transmigrating. These findings are relevant in vivo because CD157-deficient neutrophils obtained from patients with paroxysmal nocturnal hemoglobinuria are characterized by a severely impaired diapedesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Malavasi, S. Deaglio, A. Funaro, E. Ferrero, A. L. Horenstein, E. Ortolan, T. Vaisitti, and S. Aydin Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology Physiol Rev, July 1, 2008; 88(3): 841 - 886. [Abstract] [Full Text] [PDF] H. Nakayama, F. Yoshizaki, A. Prinetti, S. Sonnino, L. Mauri, K. Takamori, H. Ogawa, and K. Iwabuchi Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms J. Leukoc. Biol., March 1, 2008; 83(3): 728 - 741. [Abstract] [Full Text] [PDF]

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