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Blood, 15 December 2006, Vol. 108, No. 13, pp. 4223-4231.
Prepublished online as a Blood First Edition Paper on August 8, 2006; DOI 10.1182/blood-2006-05-024257.
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PHAGOCYTES
Receptor-type protein tyrosine phosphatase gamma (PTP ), a new identifier for myeloid dendritic cells and specialized macrophages
Daniele Lissandrini,
William Vermi,
Marzia Vezzalini,
Silvano Sozzani,
Fabio Facchetti,
Graziella Bellone,
Andrea Mafficini,
Francesca Gentili,
Maria Grazia Ennas,
Cristina Tecchio, and
Claudio Sorio
From the Department of Pathology, University of Verona, Italy; Department of Pathology, Spedali Civili, Brescia, Italy; Department of Biotechnology, Section of General Pathology and Immunology, University of Brescia and Istituto Clinico Humanitas, Rozzano, Milan, Italy; Department of Clinical Physiopathology, University of Turin, Italy; and Department of Cytomorphology, University of Cagliari, Italy.
Protein tyrosine phosphatase (PTP ) is a receptor-like molecule with a known role in murine hematopoiesis. We analyzed the regulation of PTP expression in the human hematopoietic system, where it was detected in human peripheral blood monocytes and dendritic cells (DCs) of myeloid and plasmacytoid phenotypes. Its expression was maintained during in vitro monocyte differentiation to dendritic cells (moDC) and was further increased after maturation with bacterial lipopolysaccharide (LPS), CD40L, and TNF . But PTP was absent when monocytes from the same donor were induced to differentiate in macrophages. B and T lymphocytes did not express PTP. Rather, PTP mRNA was expressed in primary and secondary lymphoid tissues, and the highest expression was in the spleen. PTP was detected by immunohistochemistry in subsets of myeloid-derived DCs and specialized macrophages (tingible bodies, sinus and alveolar macrophages). Classic macrophages in infective or reactive granulomatous reactions did not express PTP. Increased PTP expression was associated with a decreased ability to induce proliferation and interferon- secretion in T cells by moDCs from patients with advanced pancreatic cancer. Taken together, these results indicate that PTP is a finely regulated protein in DC and macrophage subsets in vitro and in vivo.
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