SEARCH:   Advanced

Blood, 15 July 2006, Vol. 108, No. 2, pp. 510-514. Prepublished online as a Blood First Edition Paper on February 28, 2006; DOI 10.1182/blood-2005-12-4835. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-12-4835v1 108/2/510    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pozgajová, M. Articles by Nieswandt, B. Search for Related Content PubMed PubMed Citation Articles by Pozgajová, M. Articles by Nieswandt, B. Related Collections Hemostasis, Thrombosis, and Vascular Biology Cell Adhesion and Motility Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Reduced thrombus stability in mice lacking the 2A-adrenergic receptor Miroslava Pogajová, Ulrich J. H. Sachs, Lutz Hein, and Bernhard Nieswandt From the Rudolf Virchow Center, Deutsche Forschungsgemeinschaft (DFG) Center for Experimental Biomedicine, the Institute of Pharmacology and Toxicology, and the Institute of Clinical Biochemistry and Pathobiochemistry, University of Würzburg, Germany. Platelet activation plays a central role in hemostasis and thrombosis. Many platelet agonists function through G-protein–coupled receptors. Epinephrine activates the 2A-adrenergic receptor (2A) that couples to Gz in platelets. Although 2A was originally cloned from platelets, its role in thrombosis and hemostasis is still unclear. Through analysis of 2A-deficient mice, variable tail bleeding times were observed. In vitro, epinephrine potentiated activation/aggregation responses of wild-type but not 2A-deficient platelets as determined by flow cytometry and aggregometry, whereas perfusion studies showed no differences in platelet adhesion and thrombus formation on collagen. To test the in vivo relevance of 2A deficiency, mice were subjected to 3 different thrombosis models. As expected, 2A-deficient mice were largely protected from lethal pulmonary thromboembolism induced by the infusion of collagen/epinephrine. In a model of FeCl3-induced injury in mesenteric arterioles, 2A–/– mice displayed a 2-fold increase in embolus formation, suggesting thrombus instability. In a third model, the aorta was mechanically injured, and blood flow was measured with an ultrasonic flow probe. In wild-type mice, all vessels occluded irreversibly, whereas in 24% of 2A-deficient mice, the initially formed thrombi embolized and blood flow was reestablished. These results demonstrate that 2A plays a significant role in thrombus stabilization. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. May, I. Hagedorn, I. Pleines, M. Bender, T. Vogtle, J. Eble, M. Elvers, and B. Nieswandt CLEC-2 is an essential platelet-activating receptor in hemostasis and thrombosis Blood, October 15, 2009; 114(16): 3464 - 3472. [Abstract] [Full Text] [PDF] J. Rivera, M. L. Lozano, L. Navarro-Nunez, and V. Vicente Platelet receptors and signaling in the dynamics of thrombus formation Haematologica, May 1, 2009; 94(5): 700 - 711. [Abstract] [Full Text] [PDF] J. M. Auger and S. P. Watson Dynamic Tyrosine Kinase-Regulated Signaling and Actin Polymerisation Mediate Aggregate Stability Under Shear Arterioscler Thromb Vasc Biol, August 1, 2008; 28(8): 1499 - 1504. [Abstract] [Full Text] [PDF] X. Su, J. Mi, J. Yan, P. Flevaris, Y. Lu, H. Liu, Z. Ruan, X. Wang, N. Kieffer, S. Chen, et al. RGT, a synthetic peptide corresponding to the integrin {beta}3 cytoplasmic C-terminal sequence, selectively inhibits outside-in signaling in human platelets by disrupting the interaction of integrin {alpha}IIb{beta}3 with Src kinase Blood, August 1, 2008; 112(3): 592 - 602. [Abstract] [Full Text] [PDF] D. Li, S. August, and D. S. Woulfe GSK3{beta} is a negative regulator of platelet function and thrombosis Blood, April 1, 2008; 111(7): 3522 - 3530. [Abstract] [Full Text] [PDF] R. J. Westrick, M. E. Winn, and D. T. Eitzman Murine Models of Vascular Thrombosis Arterioscler Thromb Vasc Biol, October 1, 2007; 27(10): 2079 - 2093. [Abstract] [Full Text] [PDF] U. J.H. Sachs and B. Nieswandt In Vivo Thrombus Formation in Murine Models Circ. Res., April 13, 2007; 100(7): 979 - 991. [Abstract] [Full Text] [PDF] C. V. Denis and D. D. Wagner Platelet Adhesion Receptors and Their Ligands in Mouse Models of Thrombosis Arterioscler Thromb Vasc Biol, April 1, 2007; 27(4): 728 - 739. [Abstract] [Full Text] [PDF] S. Offermanns Activation of Platelet Function Through G Protein-Coupled Receptors Circ. Res., December 8, 2006; 99(12): 1293 - 1304. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020