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Blood, 15 July 2006, Vol. 108, No. 2, pp. 515-517. Prepublished online as a Blood First Edition Paper on March 28, 2006; DOI 10.1182/blood-2005-11-4571. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-11-4571v1 108/2/515    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jacquemin, M. Articles by Kirkpatrick, C. J. Search for Related Content PubMed PubMed Citation Articles by Jacquemin, M. Articles by Kirkpatrick, C. J. Related Collections Hemostasis, Thrombosis, and Vascular Biology Gene Expression Free Research Articles Brief Reports Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Brief report FVIII production by human lung microvascular endothelial cells Marc Jacquemin, Arne Neyrinck, Maria Iris Hermanns, Renaud Lavend'homme, Filip Rega, Jean-Marie Saint-Remy, Kathelijne Peerlinck, Dirk Van Raemdonck, and Charles James Kirkpatrick From the Center for Molecular and Vascular Biology, University of Leuven, Belgium; Institute of Pathology, Johannes Gutenberg University, Mainz, Germany; and the Laboratory for Experimental Thoracic Surgery, Katholieke Universiteit Leuven, Belgium. While extrahepatic factor VIII (FVIII) synthesis suffices for hemostasis, the extrahepatic production sites are not well defined. We therefore investigated the ability of the human lungs to produce FVIII. Lungs from heart-beating donors who were declined for transplantation were perfused and ventilated in an isolated reperfusion model for 2 hours. A progressive accumulation of FVIII and von Willebrand factor (VWF) was recorded in the perfusion medium in 3 of 4 experiments. By contrast, factor V, fibrinogen, and immunoglobulin G (IgG) levels remained constant during the perfusion period, indicating that the accumulation of FVIII and VWF was not due to diffusion from the intercellular medium into the vascular system. Purified human lung microvascular endothelial cells produced FVIII during at least 2 passages in vitro. Altogether, these data identify the lung endothelial cells as a FVIII production site in humans. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Factor VIII biosynthesis: new inspirations? Jay Lozier Blood 2006 108: 414-415. [Full Text] [PDF] This article has been cited by other articles: N. Yadav, S. Kanjirakkuzhiyil, S. Kumar, M. Jain, A. Halder, R. Saxena, and A. Mukhopadhyay The therapeutic effect of bone marrow-derived liver cells in the phenotypic correction of murine hemophilia A Blood, November 12, 2009; 114(20): 4552 - 4561. [Abstract] [Full Text] [PDF] C. L. Madeira, M. E. Layman, R. E. de Vera, P. A. Fontes, and M. V. Ragni Extrahepatic factor VIII production in transplant recipient of hemophilia donor liver Blood, May 21, 2009; 113(21): 5364 - 5365. [Full Text] [PDF] S. F. De Meyer, H. Deckmyn, and K. Vanhoorelbeke von Willebrand factor to the rescue Blood, May 21, 2009; 113(21): 5049 - 5057. [Abstract] [Full Text] [PDF] S. L. Haberichter VWF and FVIII: the origins of a great friendship Blood, March 26, 2009; 113(13): 2873 - 2874. [Full Text] [PDF] S. Lacroix-Desmazes, A.-M. Navarrete, S. Andre, J. Bayry, S. V. Kaveri, and S. Dasgupta Dynamics of factor VIII interactions determine its immunologic fate in hemophilia A Blood, July 15, 2008; 112(2): 240 - 249. [Abstract] [Full Text] [PDF] W. C. Aird Phenotypic Heterogeneity of the Endothelium: II. Representative Vascular Beds Circ. Res., February 2, 2007; 100(2): 174 - 190. [Abstract] [Full Text] [PDF]

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