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Blood, 15 July 2006, Vol. 108, No. 2, pp. 726-733. Prepublished online as a Blood First Edition Paper on March 14, 2006; DOI 10.1182/blood-2005-10-4064. This Article Full Text Full Text (PDF) Supplemental Figure All Versions of this Article: 2005-10-4064v1 108/2/726    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Suzuki, M. Articles by Yamamoto, M. Search for Related Content PubMed PubMed Citation Articles by Suzuki, M. Articles by Yamamoto, M. Related Collections Hematopoiesis and Stem Cells Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  RED CELLS Real-time monitoring of stress erythropoiesis in vivo using Gata1 and -globin LCR luciferase transgenic mice Mikiko Suzuki, Kinuko Ohneda, Sakie Hosoya-Ohmura, Saho Tsukamoto, Osamu Ohneda, Sjaak Philipsen, and Masayuki Yamamoto From the Graduate School of Comprehensive Human Sciences, the Center for Tsukuba Advanced Research Alliance (TARA), and the Environmental Response Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, University of Tsukuba, Japan; and the Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands. Erythroid progenitors have the potential to proliferate rapidly in response to environmental stimuli. This process is referred to as stress erythropoiesis, with erythropoietin (EPO) playing central roles in its promotion. In this study, we wanted to elucidate the molecular mechanisms governing the regulation of stress erythropoiesis and the maintenance of red-cell homeostasis. This was achieved by our development of a noninvasive real-time monitoring system for erythropoiesis using transgenic mouse lines expressing luciferase under the control of the mouse Gata1 hematopoietic regulatory domain (G1-HRD-luc) or human -globin locus control region (Hbb-LCR-luc). Optical bioluminescence images revealed that the luciferase was specifically expressed in spleen and bone marrow and was induced rapidly in response to anemia and hypoxia stimuli. The G1-HRD-luc activity tracked the emergence and disappearance of proerythroblast-stage progenitors, whereas the Hbb-LCR-luc activity tracked erythroblasts and later stage erythroid cells. Increased plasma EPO concentration preceded an increase in G1-HRD-luc, supporting our contention that EPO acts as the key upstream signal in stress erythropoiesis. Hence, we conclude that G1-HRD-luc and Hbb-LCR-luc reporters are differentially activated during stress erythropoiesis and that the transgenic mouse lines used serve as an important means for understanding the homeostatic regulation of erythropoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Ohneda, S. Ohmori, Y. Ishijima, M. Nakano, and M. Yamamoto Characterization of a Functional ZBP-89 Binding Site That Mediates Gata1 Gene Expression during Hematopoietic Development J. Biol. Chem., October 30, 2009; 284(44): 30187 - 30199. [Abstract] [Full Text] [PDF] M. Suzuki, T. Moriguchi, K. Ohneda, and M. Yamamoto Differential Contribution of the Gata1 Gene Hematopoietic Enhancer to Erythroid Differentiation Mol. Cell. Biol., March 1, 2009; 29(5): 1163 - 1175. [Abstract] [Full Text] [PDF] T. Yamashita, O. Ohneda, A. Sakiyama, F. Iwata, K. Ohneda, and Y. Fujii-Kuriyama The microenvironment for erythropoiesis is regulated by HIF-2{alpha} through VCAM-1 in endothelial cells Blood, August 15, 2008; 112(4): 1482 - 1492. [Abstract] [Full Text] [PDF] J. Fang, M. Menon, D. Zhang, B. Torbett, L. Oxburgh, M. Tschan, E. Houde, and D. M. Wojchowski Attenuation of EPO-dependent erythroblast formation by death-associated protein kinase-2 Blood, August 1, 2008; 112(3): 886 - 890. [Abstract] [Full Text] [PDF] R. Antia, A. Yates, and J. C de Roode The dynamics of acute malaria infections. I. Effect of the parasite's red blood cell preference Proc R Soc B, June 22, 2008; 275(1641): 1449 - 1458. [Abstract] [Full Text] [PDF] V. C Barclay, L. Raberg, B. H.K Chan, S. Brown, D. Gray, and A. F Read CD4+T cells do not mediate within-host competition between genetically diverse malaria parasites Proc R Soc B, May 22, 2008; 275(1639): 1171 - 1179. [Abstract] [Full Text] [PDF] L. Gutierrez, S. Tsukamoto, M. Suzuki, H. Yamamoto-Mukai, M. Yamamoto, S. Philipsen, and K. Ohneda Ablation of Gata1 in adult mice results in aplastic crisis, revealing its essential role in steady-state and stress erythropoiesis Blood, April 15, 2008; 111(8): 4375 - 4385. [Abstract] [Full Text] [PDF] M. Kobayashi and M. Yamamoto Regulation of GATA1 Gene Expression J. Biochem., July 1, 2007; 142(1): 1 - 10. [Abstract] [Full Text] [PDF]

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