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Blood, 15 August 2006, Vol. 108, No. 4, pp. 1406-1412. Prepublished online as a Blood First Edition Paper on April 13, 2006; DOI 10.1182/blood-2005-11-012864. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2005-11-012864v1 108/4/1406    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lilleri, D. Articles by Locatelli, F. Search for Related Content PubMed PubMed Citation Articles by Lilleri, D. Articles by Locatelli, F. Related Collections Immunobiology Transplantation Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Prospective simultaneous quantification of human cytomegalovirus-specific CD4+ and CD8+ T-cell reconstitution in young recipients of allogeneic hematopoietic stem cell transplants Daniele Lilleri, Giuseppe Gerna, Chiara Fornara, Laura Lozza, Rita Maccario, and Franco Locatelli From the Servizio di Virologia, Unità di Oncoematologia Pediatrica, and Laboratori Sperimentali di Ricerca, Area Trapiantologica, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy. We investigated immune reconstitution against human cytomegalovirus (HCMV) in 57 hematopoietic stem cell transplant (HSCT) recipients, aged 1 to 24 years, through a novel method combining T-cell stimulation by HCMV-infected autologous dendritic cells with simultaneous cytometric quantification of HCMV-specific, IFN-producing CD4+ and CD8+ T cells. Lymphoproliferative response (LPR) to HCMV antigens was also determined. Patients were stratified into 2 groups according to HCMV serostatus, comprising 39 HCMV-seropositive (R+) and 18 HCMV-seronegative (R–) patients who received a transplant from a sero-positive donor. Recovery of both HCMV-specific CD4+ and CD8+ T-cell immunity occurred in all 39 R+ patients within 6 months and in 6 (33%) of 18 R– patients within 12 months. In R+ patients, the median numbers of HCMV-specific CD8+ and CD4+T cells were significantly higher than those of healthy controls, starting from days +60 and +180, respectively. In R– patients, the median numbers of HCMV-specific T cells were consistently lower than in R+ patients. LPR was delayed compared with reconstitution of IFN-producing T cells. Patients with delayed specific immune reconstitution experienced recurrent episodes of HCMV infection. HCMV seropositivity of young HSCT recipients is the major factor responsible for HCMV-specific immune reconstitution, irrespective of donor serostatus, and measurement of HCMV-specific T cells appears useful for correct management of HCMV infection. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Weinberg, J. Spritzler, M. Nokta, R. Schrier, A. Landay, D. Brown, R. Pollard, and for the ACTG CMV Task Force In Vitro Cell-Mediated Immune Responses of Human Immunodeficiency Virus-Infected and -Uninfected Individuals to Whole Cytomegalovirus Antigens and Their Subunits Clin. Vaccine Immunol., September 1, 2008; 15(9): 1398 - 1409. [Abstract] [Full Text] [PDF] C. Solano, I. Benet, M. A. Clari, J. Nieto, R. de la Camara, J. Lopez, J. C. Hernandez-Boluda, M. J. Remigia, I. Jarque, M. L. Calabuig, et al. Enumeration of cytomegalovirus-specific interferon{gamma} CD8+ and CD4+ T cells early after allogeneic stem cell transplantation may identify patients at risk of active cytomegalovirus infection Haematologica, September 1, 2008; 93(9): 1434 - 1436. [Full Text] [PDF] G. Gerna, A. Sarasini, M. Patrone, E. Percivalle, L. Fiorina, G. Campanini, A. Gallina, F. Baldanti, and M. G. Revello Human cytomegalovirus serum neutralizing antibodies block virus infection of endothelial/epithelial cells, but not fibroblasts, early during primary infection J. Gen. Virol., April 1, 2008; 89(4): 853 - 865. [Abstract] [Full Text] [PDF] D. Lilleri, G. Gerna, M. Furione, M. E. Bernardo, G. Giorgiani, S. Telli, F. Baldanti, and F. Locatelli Use of a DNAemia cut-off for monitoring human cytomegalovirus infection reduces the number of preemptively treated children and young adults receiving hematopoietic stem-cell transplantation compared with qualitative pp65 antigenemia Blood, October 1, 2007; 110(7): 2757 - 2760. [Abstract] [Full Text] [PDF]

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