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Blood, 1 September 2006, Vol. 108, No. 5, pp. 1652-1660. Prepublished online as a Blood First Edition Paper on May 2, 2006; DOI 10.1182/blood-2006-02-002030. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-02-002030v1 108/5/1652    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lacout, C. Articles by Villeval, J.-L. Search for Related Content PubMed PubMed Citation Articles by Lacout, C. Articles by Villeval, J.-L. Related Collections Hematopoiesis and Stem Cells Neoplasia Oncogenes and Tumor Suppressors Related Articles in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA JAK2V617F expression in murine hematopoietic cells leads to MPD mimicking human PV with secondary myelofibrosis Catherine Lacout, Didier F. Pisani, Micheline Tulliez, Françoise Moreau Gachelin, William Vainchenker, and Jean-Luc Villeval From the Institut National de la Sante et de la Recherche Medicale (INSERM) U790, Université Paris XI, Institut Gustave Roussy (IGR), Villejuif, France; Hôpital Cochin, Université Paris V, Paris, France; and INSERM U528, Institut Curie, Université Paris V, Paris, France. A JAK2V617F mutation is frequently found in several BCR/ABL-negative myeloproliferative disorders. To address the contribution of this mutant to the pathogenesis of these different myeloproliferative disorders, we used an adoptive transfer of marrow cells transduced with a retrovirus expressing JAK2V617F in recipient irradiated mice. Hosts were analyzed during the 6 months after transplantation. For a period of 3 months, mice developed polycythemia, macrocytosis and usually peripheral blood granulocytosis. Transient thrombocytosis was only observed in a low-expresser group. All mice displayed trilineage hyperplasia in marrow and spleen along with an amplification of myeloid and erythroid progenitor cells and a formation of endogenous erythroid colonies. After 3 to 4 months, polycythemia regressed, abnormally shaped red blood cells and platelets were seen in circulation, and a deposition of reticulin fibers was observed in marrow and spleen. Development of fibrosis was associated with anemia, thrombocytopenia, high neutrophilia, and massive splenomegaly. These features mimic human polycythemia vera and its evolution toward myelofibrosis. This work demonstrates that JAK2V617F is sufficient for polycythemia and fibrosis development and offers an in vivo model to assess novel therapeutic approaches for JAK2V617F-positive pathologies. Questions remain regarding the exact contribution of JAK2V617F in other myeloproliferative disorders. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Articles in Blood Online: JAK2V617F: prevalence in a large Chinese hospital population Xuesong Xu, Qi Zhang, Jian Luo, Shu Xing, Qingshan Li, Sanford B. Krantz, Xueqi Fu, and Zhizhuang Joe Zhao Blood 2007 109: 339-342. [Abstract] [Full Text] [PDF] Animal models of the MPD: lack of the clones Jerry L. Spivak Blood 2006 108: 1427-1428. [Full Text] [PDF] This article has been cited by other articles: I. Plo, M. Nakatake, L. Malivert, J.-P. de Villartay, S. Giraudier, J.-L. Villeval, L. Wiesmuller, and W. 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