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Blood, 15 September 2006, Vol. 108, No. 6, pp. 1877-1886. Prepublished online as a Blood First Edition Paper on May 11, 2006; DOI 10.1182/blood-2006-04-014894. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-04-014894v1 108/6/1877    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rolny, C. Articles by Claesson-Welsh, L. Search for Related Content PubMed PubMed Citation Articles by Rolny, C. Articles by Claesson-Welsh, L. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Platelet-derived growth factor receptor- promotes early endothelial cell differentiation Charlotte Rolny, Ingrid Nilsson, Peetra Magnusson, Annika Armulik, Lars Jakobsson, Parri Wentzel, Per Lindblom, Jenny Norlin, Christer Betsholtz, Rainer Heuchel, Michael Welsh, and Lena Claesson-Welsh From the Department of Genetics and Pathology and the Department of Medical Cell Biology, Uppsala University; the Department of Medical Biochemistry and Biophysics and the Department of Medicine, Karolinska Institute, Stockholm; and the Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden. Platelet-derived growth factor BB (PDGF-BB) has been assigned a critical role in vascular stability by promoting the recruitment of PDGF receptor-–expressing perivascular cells. Here we present data indicating that early hematopoietic/endothelial (hemangio) precursors express PDGFR- based on coexpression with CD31, vascular endothelial growth factor receptor-2, and CD41 in 2 models: mouse yolk sac (embryonic day 8 [E8]) and differentiating mouse embryonic stem cells (embryoid bodies). Expression of PDGFR- on hemangioprecursor cells in the embryoid bodies gradually disappeared, and, at E14, expression appeared on perivascular cells. Activation of the PDGFR- on the hemangioprecursors accelerated the differentiation of endothelial cells, whereas differentiation of the hematopoietic lineage was suppressed. In E9.5 yolk sacs derived from recombinant mice expressing kinase-active PDGFR- with an aspartic acid to asparagine (D894N) replacement in the kinase activating loop and from mice with ubiquitous expression of PDGF-BB driven by the Rosa26 locus, the number of CD41-expressing early hematopoietic cells decreased by 36% and 34%, respectively, compared with staged wild-type littermates. Moreover, enhanced vascular remodeling was evident in the Rosa26–PDGF-BB yolk sacs. We conclude that PDGFR- is expressed on early hemangioprecursor cells, regulating vascular/hematopoietic development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: PDGFR: a multifaceted player in vascular and hematopoietic development Zhenping Zhu Blood 2006 108: 1788-1789. [Full Text] [PDF] This article has been cited by other articles: A. M. L. Coluccia, T. Cirulli, P. Neri, D. Mangieri, M. C. Colanardi, A. Gnoni, N. Di Renzo, F. Dammacco, P. Tassone, D. Ribatti, et al. Validation of PDGFR{beta} and c-Src tyrosine kinases as tumor/vessel targets in patients with multiple myeloma: preclinical efficacy of the novel, orally available inhibitor dasatinib Blood, August 15, 2008; 112(4): 1346 - 1356. [Abstract] [Full Text] [PDF] J. Andrae, R. Gallini, and C. Betsholtz Role of platelet-derived growth factors in physiology and medicine Genes & Dev., May 15, 2008; 22(10): 1276 - 1312. [Abstract] [Full Text] [PDF] D. Mottet, A. Bellahcene, S. Pirotte, D. Waltregny, C. Deroanne, V. Lamour, R. Lidereau, and V. Castronovo Histone Deacetylase 7 Silencing Alters Endothelial Cell Migration, a Key Step in Angiogenesis Circ. Res., December 7, 2007; 101(12): 1237 - 1246. [Abstract] [Full Text] [PDF] L. Jakobsson, J. Kreuger, and L. Claesson-Welsh Building blood vessels--stem cell models in vascular biology J. Cell Biol., June 21, 2007; 177(5): 751 - 755. [Abstract] [Full Text] [PDF] H. Uusitalo-Jarvinen, T. Kurokawa, B. M. Mueller, P. Andrade-Gordon, M. Friedlander, and W. Ruf Role of Protease Activated Receptor 1 and 2 Signaling in Hypoxia-Induced Angiogenesis Arterioscler. Thromb. Vasc. Biol., June 1, 2007; 27(6): 1456 - 1462. [Abstract] [Full Text] [PDF] P. Dentelli, A. Rosso, A. Balsamo, S. Colmenares Benedetto, A. Zeoli, M. Pegoraro, G. Camussi, L. Pegoraro, and M. F. Brizzi C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium Blood, May 15, 2007; 109(10): 4264 - 4271. [Abstract] [Full Text] [PDF]

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