SEARCH:   Advanced

Blood, 15 September 2006, Vol. 108, No. 6, pp. 1957-1964. Prepublished online as a Blood First Edition Paper on May 16, 2006; DOI 10.1182/blood-2006-03-010918. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-03-010918v1 108/6/1957    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Groot, F. Articles by de Jong, E. C. Search for Related Content PubMed PubMed Citation Articles by Groot, F. Articles by de Jong, E. C. Related Collections Immunobiology Phagocytes Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Opposing roles of blood myeloid and plasmacytoid dendritic cells in HIV-1 infection of T cells: transmission facilitation versus replication inhibition Fedde Groot, Toni M. M. van Capel, Martien L. Kapsenberg, Ben Berkhout, and Esther C. de Jong From the Department of Human Retrovirology, the Department of Cell Biology and Histology, and the Department of Dermatology, Academic Medical Center, University of Amsterdam, The Netherlands. CD11c+ myeloid dendritic cells (MDCs) and CD11c– CD123+ plasmacytoid DCs (PDCs) have been identified as main human DC subsets. MDCs are professional antigen-presenting cells for T cells, and include Langerhans cells, dermal DCs, and interstitial DCs. They have been associated with HIV-1 capture and sexual transmission, whereas PDCs play an important role in the innate immune responses to different types of viruses, including HIV-1. To compare the influence of MDCs and PDCs on HIV-1 infection of T cells, we isolated donor-matched MDCs and PDCs from peripheral blood, activated them by adding different maturation-inducing compounds, and cocultured them with T cells and HIV-1. We found that MDCs enhance HIV-1 infection through capture of the virus and subsequent transmission to T cells, and that differently matured MDC subsets have different HIV-1 transmission efficiencies. These differences were not due to soluble factors, viral capture differences, or the expression of integrins ICAM-1, -2, -3, or LFA-1. In contrast, regardless of their state of maturation, PDCs inhibit HIV-1 replication in T cells through the secretion of IFN and an additional, unidentified small molecule. This study shows that the 2 main types of DCs have opposing roles in HIV-1 infection of T cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Opposing roles of dendritic cell subsets in HIV-1 infection Justin Stebbing and Mark Bower Blood 2006 108: 1785-1786. [Full Text] [PDF] This article has been cited by other articles: D. Piccioli, C. Sammicheli, S. Tavarini, S. Nuti, E. Frigimelica, A. G.O. Manetti, A. Nuccitelli, S. Aprea, S. Valentini, E. Borgogni, et al. Human plasmacytoid dendritic cells are unresponsive to bacterial stimulation and require a novel type of cooperation with myeloid dendritic cells for maturation Blood, April 30, 2009; 113(18): 4232 - 4239. [Abstract] [Full Text] [PDF] B. Liu, A. M. Woltman, H. L. A. Janssen, and A. Boonstra Modulation of dendritic cell function by persistent viruses J. Leukoc. Biol., February 1, 2009; 85(2): 205 - 214. [Abstract] [Full Text] [PDF] S. C. Barry and P. T. H. Coates Retroviral escape by Friendly infected DCs Blood, December 1, 2007; 110(12): 3819 - 3820. [Full Text] [PDF] C. Tomescu, J. Chehimi, V. C. Maino, and L. J. Montaner NK Cell Lysis of HIV-1-Infected Autologous CD4 Primary T Cells: Requirement for IFN-Mediated NK Activation by Plasmacytoid Dendritic Cells J. Immunol., August 15, 2007; 179(4): 2097 - 2104. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020