SEARCH:   Advanced

Blood First Edition Paper, prepublished online October 5, 2006; DOI 10.1182/blood-2006-04-018564. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-04-018564v1 108/8/2857 blood-2006-04-018564v2    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gavina, M. Articles by Torrente, Y. Search for Related Content PubMed PubMed Citation Articles by Gavina, M. Articles by Torrente, Y. Related Collections Cell Adhesion and Motility Stem Cells in Hematology Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  STEM CELLS IN HEMATOLOGY VCAM-1 expression on dystrophic muscle vessels has a critical role in the recruitment of human blood-derived CD133+ stem cells after intra-arterial transplantation Manuela Gavina, Marzia Belicchi, Barbara Rossi, Linda Ottoboni, Fabio Colombo, Mirella Meregalli, Maurizio Battistelli, Laura Forzenigo, Piero Biondetti, Federica Pisati, Daniele Parolini, Andrea Farini, Andrew C. Issekutz, Nereo Bresolin, Franco Rustichelli, Gabriela Constantin, and Yvan Torrente From the Stem Cell Laboratory, Department of Neurological Sciences, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Italy; the Department of Pathology, Division of General Pathology, University of Verona, Italy; the Fondazione IRCCS Ospedale Maggiore Policlinico, Institute of Nuclear Medicine, Milan, Italy; the Radiology Unit, Fondazione IRCCS Ospedale Maggiore Policlinico of Milan, Italy; the Department of Pediatrics, Pathology, and Microbiology-Immunology, Dalhousie University, Halifax, NS, Canada; and the Department of Sciences Applied to Complex Systems, Polytechnic University of Marche, Ancona, Italy. Recently our group demonstrated the myogenic capacity of human CD133+ cells isolated from peripheral blood when delivered in vivo through the arterial circulation into the muscle of dystrophic scid/mdx mice. CD133+ stem cells express the adhesion molecules CD44, LFA-1, PSGL-1, 4-integrins, L-selectin, and chemokine receptor CCR7. Moreover these cells adhere in vitro to VCAM-1 spontaneously and after stimulation with CCL19. Importantly, after muscle exercise, we found that the expression of VCAM-1 is strongly up-regulated in dystrophic muscle vessels, whereas the number of rolling and firmly adhered CD133+ stem cells significantly increased. Moreover, human dystrophin expression was significantly increased when muscle exercise was performed 24 hours before the intra-arterial injection of human CD133+ cells. Finally, treatment of exercised dystrophic mice with anti-VCAM-1 antibodies led to a dramatic blockade of CD133+ stem cell migration into the dystrophic muscle. Our results show for the first time that the expression of VCAM-1 on dystrophic muscle vessels induced by exercise controls muscle homing of human CD133+ stem cells, opening new perspectives for a potential therapy of muscular dystrophy based on the intra-arterial delivery of CD133+ stem cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I.-Y. Oh, C.-H. Yoon, J. Hur, J.-H. Kim, T.-Y. Kim, C.-S. Lee, K.-W. Park, I.-H. Chae, B.-H. Oh, Y.-B. Park, et al. Involvement of E-selectin in recruitment of endothelial progenitor cells and angiogenesis in ischemic muscle Blood, December 1, 2007; 110(12): 3891 - 3899. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020