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Blood, 1 November 2006, Vol. 108, No. 9, pp. 3053-3060. Prepublished online as a Blood First Edition Paper on July 6, 2006; DOI 10.1182/blood-2006-05-020495. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-05-020495v1 108/9/3053    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hoffman, M. Articles by Monroe, D. M. Search for Related Content PubMed PubMed Citation Articles by Hoffman, M. Articles by Monroe, D. M. Related Collections Free Research Articles Hemostasis, Thrombosis, and Vascular Biology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Cutaneous wound healing is impaired in hemophilia B Maureane Hoffman, Anna Harger, Angela Lenkowski, Ulla Hedner, Harold R. Roberts, and Dougald M. Monroe From the Department of Pathology, Duke University, Durham, NC; Lab Service Durham Veterans Affairs Medical Center, NC; University Of North Carolina Medical Center Department of Medicine, Chapel Hill, NC; and the Department of Research and Development, Novo Nordisk A/S, Maalov, Denmark. We used a mouse model to test the hypothesis that the time course and histology of wound healing is altered in hemophilia B. Punch biopsies (3 mm) were placed in the skin of normal mice and mice with hemophilia. The size of the wounds was measured daily until the epidermal defect closed. All wounds closed in mice with hemophilia by 12 days, compared with 10 days in normal animals. Skin from the area of the wound was harvested at different time points and examined histologically. Hemophilic animals developed subcutaneous hematomas; normal animals did not. Macrophage infiltration was significantly delayed in hemophilia B. Unexpectedly, hemophilic mice developed twice as many blood vessels in the healing wounds as controls, and the increased vascularity persisted for at least 2 weeks. The deposition and persistence of ferric iron was also greater in hemophilic mice. We hypothesize that iron plays a role in promoting excess angiogenesis after wounding as it had been proposed to do in hemophilic arthropathy. We have demonstrated that impaired coagulation leads to delayed wound healing with abnormal histology. Our findings have significant implications for treatment of patients with hemophilia, and also highlight the importance of rapidly establishing hemostasis following trauma or surgery. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Heavy metal FIX for Christmas wounds Leonard A. Valentino Blood 2006 108: 2888. [Full Text] [PDF] This article has been cited by other articles: T. Orfeo, K. E. Brummel-Ziedins, M. Gissel, S. Butenas, and K. G. Mann The Nature of the Stable Blood Clot Procoagulant Activities J. Biol. Chem., April 11, 2008; 283(15): 9776 - 9786. [Abstract] [Full Text] [PDF] A. G. McDonald, K. Yang, H. R. Roberts, D. M. Monroe, and M. Hoffman Perivascular tissue factor is down-regulated following cutaneous wounding: implications for bleeding in hemophilia Blood, February 15, 2008; 111(4): 2046 - 2048. [Abstract] [Full Text] [PDF] Y. Zheng, M. Watanabe, T. Kuraishi, S. Hattori, C. Kai, and M. Shibuya Chimeric VEGF-ENZ7/PlGF Specifically Binding to VEGFR-2 Accelerates Skin Wound Healing via Enhancement of Neovascularization Arterioscler. Thromb. Vasc. Biol., March 1, 2007; 27(3): 503 - 511. [Abstract] [Full Text] [PDF]

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