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 Blood, 1 January 2007, Vol. 109, No. 1, pp. 343-352.
Prepublished online as a Blood First Edition Paper on August 29, 2006; DOI 10.1182/blood-2006-03-006569.

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RED CELLS

Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell-surface antigen expression

Stuart T. Fraser1, Joan Isern1, and Margaret H. Baron1,5,

1 Department of Medicine, Mount Sinai School of Medicine, New York, NY; 2 Department of Molecular, Cellular, and Developmental Biology, Mount Sinai School of Medicine, New York, NY; 3 Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY; 4 Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY; and 5 Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, NY

Primitive erythroblasts (EryPs) are the first hematopoietic cell type to form during mammalian embryogenesis and emerge within the blood islands of the yolk sac. Large, nucleated EryPs begin to circulate around midgestation, when connections between yolk sac and embryonic vasculature mature. Two to 3 days later, small cells of the definitive erythroid lineage (EryD) begin to differentiate within the fetal liver and rapidly outnumber EryPs in the circulation. The development and maturation of EryPs remain poorly defined. Our analysis of embryonic blood at different stages reveals a stepwise developmental progression within the EryP lineage from E9.5 to E12.5. Thereafter, EryDs are also present in the bloodstream, and the 2 lineages are not easily distinguished. We have generated a transgenic mouse line in which the human {epsilon}-globin gene promoter drives expression of green fluorescent protein exclusively within the EryP lineage. Here, we have used this line to characterize changes in cell morphology and surface-marker expression as EryPs mature and to track EryP numbers and enucleation throughout gestation. This study identifies previously unrecognized synchronous developmental stages leading to the maturation of EryPs in the mouse embryo. Unexpectedly, we find that EryPs are a stable cell population that persists through the end of gestation.


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