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Blood, 1 January 2007, Vol. 109, No. 1, pp. 65-70. Prepublished online as a Blood First Edition Paper on September 12, 2006; DOI 10.1182/blood-2006-04-016741. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-04-016741v1 109/1/65    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kiem, H.-P. Articles by Russell, D. W. Search for Related Content PubMed PubMed Citation Articles by Kiem, H.-P. Articles by Russell, D. W. Related Collections Stem Cells in Hematology Gene Therapy Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  GENE THERAPY Foamy virus–mediated gene transfer to canine repopulating cells Hans-Peter Kiem1,3,, James Allen2, Grant Trobridge1,2, Erik Olson2, Kirsten Keyser1, Laura Peterson1, and David W. Russell2,4 1 Clinical Division, Fred Hutchinson Cancer Research Center, Seattle WA; 2 Department of Medicine, University of Washington School of Medicine, Seattle WA; 3 Department of Pathology, University of Washington School of Medicine, Seattle WA; and 4 Department of Biochemistry, University of Washington School of Medicine, Seattle WA Foamy virus (FV) vectors are particularly attractive gene-transfer vectors for stem-cell gene therapy because they form a stable transduction intermediate in quiescent cells and can efficiently transduce hematopoietic stem cells. Here, we studied the use of FV vectors to transduce long-term hematopoietic repopulating cells in the dog, a clinically relevant large animal model. Mobilized canine peripheral blood (PB) CD34+ cells were transduced with an enhanced green fluorescent protein (EGFP)–expressing FV vector in an 18-hour transduction protocol. All 3 dogs studied had rapid neutrophil engraftment to greater than 500/µL with a median of 10 days. Transgene expression was detected in all cell lineages (B cells, T cells, granulocytes, red blood cells, and platelets), indicating multilineage engraftment of transduced cells. Up to 19% of blood cells were EGFP+, and this was confirmed at the DNA level by real-time polymerase chain reaction (PCR) and Southern blot analysis. These transduction rates were higher than the best results we obtained previously with lentiviral vectors in a similar transduction protocol. Integration site analysis also demonstrated polyclonal repopulation and the transduction of multipotential hematopoietic repopulating cells. These data suggest that FV vectors should be useful for stem-cell gene therapy, particularly for applications in which short transduction protocols are critical. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Bozorgmehr, S. Laufs, S. E. Sellers, I. Roeder, W. J. Zeller, C. E. Dunbar, and S. Fruehauf No Evidence of Clonal Dominance in Primates up to 4 Years Following Transplantation of Multidrug Resistance 1 Retrovirally Transduced Long-Term Repopulating Cells Stem Cells, October 1, 2007; 25(10): 2610 - 2618. [Abstract] [Full Text] [PDF]

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