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Blood, 15 May 2007, Vol. 109, No. 10, pp. 4343-4350. Prepublished online as a Blood First Edition Paper on January 18, 2007; DOI 10.1182/blood-2006-05-025940. This Article Full Text Full Text (PDF) Supplemental Tables and Figure All Versions of this Article: blood-2006-05-025940v1 109/10/4343    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by De Fanis, U. Articles by Casolaro, V. Search for Related Content PubMed PubMed Citation Articles by De Fanis, U. Articles by Casolaro, V. Related Collections Immunobiology Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells Umberto De Fanis1, Francesca Mori1, Rebecca J. Kurnat1, Won Kyung Lee1, Maria Bova1, N. Franklin Adkinson1, and Vincenzo Casolaro1 1 Division of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, Baltimore, MD GATA-3 and T-box expressed in T cells (T-bet) play central roles in Th-cell development and function. Consistently, studies in mice document their selective expression in Th1 and Th2 cells, respectively. In contrast, it is not clear whether these genes are regulated in human Th cells. Here we show that T-bet expression is polarized to a comparable degree in human and mouse Th-cell cultures, while only mouse GATA3 is subject to substantial regulation. This did not reflect differential skewing efficiency in human versus mouse cultures, as these contained similar frequencies of IFN-– and IL-4–producing cells. However, GATA-3 was expressed at significantly higher levels in human IL-4–producing cells enriched via capture with monoclonal antibodies (mAbs) against the PGD2 receptor, CRTH2, the best selective Th2-cell surface marker to date. Along with increased IL-4 and GATA-3, CRTH2+ Th cells isolated from Th2-skewed cultures or the circulating memory pool exhibited markedly decreased IFN- and T-bet expression. Thus, the human GATA-3 gene is not regulated in response to polarizing signals that are sufficient to direct Th2-specific expression in mouse cells. This postulates the involvement of an additional level of complexity in the regulation of human GATA-3 expression and stresses the existence of nontrivial differences in the regulation of human versus mouse T-cell function. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Hashimoto-Okada, T. Kitawaki, N. Kadowaki, S. Iwata, C. Morimoto, T. Hori, and T. Uchiyama The CD70-CD27 interaction during the stimulation with dendritic cells promotes naive CD4+ T cells to develop into T cells producing a broad array of immunostimulatory cytokines in humans Int. Immunol., June 25, 2009; (2009) dxp056v1. [Abstract] [Full Text] [PDF] J. Tahvanainen, T. Kallonen, H. Lahteenmaki, K. M. Heiskanen, J. Westermarck, K. V. S. Rao, and R. Lahesmaa PRELI is a mitochondrial regulator of human primary T-helper cell apoptosis, STAT6, and Th2-cell differentiation Blood, February 5, 2009; 113(6): 1268 - 1277. [Abstract] [Full Text] [PDF]

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