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Blood, 1 June 2007, Vol. 109, No. 11, pp. 4761-4768. Prepublished online as a Blood First Edition Paper on February 27, 2007; DOI 10.1182/blood-2006-12-062471. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-12-062471v1 109/11/4761    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Melero-Martin, J. M. Articles by Bischoff, J. Search for Related Content PubMed PubMed Citation Articles by Melero-Martin, J. M. Articles by Bischoff, J. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Stem Cells in Hematology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY In vivo vasculogenic potential of human blood-derived endothelial progenitor cells Juan M. Melero-Martin1, Zia A. Khan1, Arnaud Picard1, Xiao Wu1, Sailaja Paruchuri1, and Joyce Bischoff1 1 Vascular Biology Program and Department of Surgery, Children's Hospital, Boston, Harvard Medical School, Boston, MA Vascularization of tissues is a major challenge of tissue engineering (TE). We hypothesize that blood-derived endothelial progenitor cells (EPCs) have the required proliferative and vasculogenic activity to create vascular networks in vivo. To test this, EPCs isolated from human umbilical cord blood or from adult peripheral blood, and human saphenous vein smooth muscle cells (HSVSMCs) as a source of perivascular cells, were combined in Matrigel and implanted subcutaneously into immunodeficient mice. Evaluation of implants at one week revealed an extensive network of human-specific lumenal structures containing erythrocytes, indicating formation of functional anastomoses with the host vasculature. Quantitative analyses showed the microvessel density was significantly superior to that generated by human dermal microvascular endothelial cells (HDMECs) but similar to that generated by human umbilical vein endothelial cells (HUVECs). We also found that as EPCs were expanded in culture, their morphology, growth kinetics, and proliferative responses toward angiogenic factors progressively resembled those of HDMECs, indicating a process of in vitro maturation. This maturation correlated with a decrease in the degree of vascularization in vivo, which could be compensated for by increasing the number of EPCs seeded into the implants. Our findings strongly support the use of human EPCs to form vascular networks in engineered organs and tissues. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals Mervin C. Yoder, Laura E. Mead, Daniel Prater, Theresa R. Krier, Karim N. Mroueh, Fang Li, Rachel Krasich, Constance J. Temm, Josef T. Prchal, and David A. Ingram Blood 2007 109: 1801-1809. [Abstract] [Full Text] [PDF] This article has been cited by other articles: J. M. Melero-Martin, M. E. De Obaldia, S.-Y. Kang, Z. A. Khan, L. Yuan, P. Oettgen, and J. Bischoff Engineering Robust and Functional Vascular Networks In Vivo With Human Adult and Cord Blood-Derived Progenitor Cells Circ. Res., July 18, 2008; 103(2): 194 - 202. [Abstract] [Full Text] [PDF] M. Thill, N. V. Strunnikova, M. J. Berna, N. Gordiyenko, K. Schmid, S. W. Cousins, D. J. S. Thompson, and K. G. Csaky Late Outgrowth Endothelial Progenitor Cells in Patients with Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2696 - 2708. [Abstract] [Full Text] [PDF] R. Ria, C. Piccoli, T. Cirulli, F. Falzetti, G. Mangialardi, D. Guidolin, A. Tabilio, N. Di Renzo, A. Guarini, D. Ribatti, et al. Endothelial Differentiation of Hematopoietic Stem and Progenitor Cells from Patients with Multiple Myeloma Clin. Cancer Res., March 15, 2008; 14(6): 1678 - 1685. [Abstract] [Full Text] [PDF] D. A. Ingram, I. Z. Lien, L. E. Mead, M. Estes, D. N. Prater, E. Derr-Yellin, L. A. DiMeglio, and L. S. Haneline In Vitro Hyperglycemia or a Diabetic Intrauterine Environment Reduces Neonatal Endothelial Colony-Forming Cell Numbers and Function Diabetes, March 1, 2008; 57(3): 724 - 731. [Abstract] [Full Text] [PDF] P. Au, L. M. Daheron, D. G. Duda, K. S. Cohen, J. A. Tyrrell, R. M. Lanning, D. Fukumura, D. T. Scadden, and R. K. Jain Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels Blood, February 1, 2008; 111(3): 1302 - 1305. [Abstract] [Full Text] [PDF] X. Wu, M. W. Lensch, J. Wylie-Sears, G. Q. Daley, and J. Bischoff Hemogenic Endothelial Progenitor Cells Isolated from Human Umbilical Cord Blood Stem Cells, November 1, 2007; 25(11): 2770 - 2776. [Abstract] [Full Text] [PDF]

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