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 Blood, 1 June 2007, Vol. 109, No. 11, pp. 4806-4809.
Prepublished online as a Blood First Edition Paper on February 20, 2007; DOI 10.1182/blood-2006-09-047449.

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HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY

Brief Report

The novel inhibitory receptor G6B is expressed on the surface of platelets and attenuates platelet function in vitro

Stephen A. Newland1, Iain C. Macaulay2, Andres R. Floto1, Edwin C. de Vet3, Willem H. Ouwehand2, Nicholas A. Watkins2, Paul A. Lyons1, and Duncan R. Campbell4

1 Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom; 2 National Blood Service Cambridge and Department of Haematology, University of Cambridge, Cambridge, United Kingdom; 3 MRC Rosalind Franklin Centre for Genomics Research, Hinxton, Cambridge, United Kingdom; 4 Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom

The G6B cell-surface receptor, which contains a single Ig-like domain, has been shown to bind to SHP-1 and SHP-2 after phosphorylation of 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic tail, classifying this protein as a new member of the family of inhibitory receptors. In this study, we demonstrate by real-time polymerase chain reaction (PCR) and Western-blot analysis that G6B is expressed on platelets. Cross-linking of G6B with polyclonal antisera has a significant inhibitory effect on platelet aggregation and activation by agonists such as ADP and collagen-related peptide (CRP). This inhibitory function of G6B appears to operate in a calcium-independent manner. Our results suggest that G6B represents a novel inhibitory receptor found on the surface of platelets and that it could be an antithrombotic drug target.


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