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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5215-5222. Prepublished online as a Blood First Edition Paper on February 27, 2007; DOI 10.1182/blood-2006-10-055350. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-10-055350v1 109/12/5215    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Meyer, L. Articles by Verdier, F. Search for Related Content PubMed PubMed Citation Articles by Meyer, L. Articles by Verdier, F. Related Collections Hematopoiesis and Stem Cells Cell Cycle Signal Transduction Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS ß-Trcp mediates ubiquitination and degradation of the erythropoietin receptor and controls cell proliferation Laure Meyer1,3,5, Bénédicte Deau1,3,5, Hana Forejtníková1,3,5, Dominique Duménil1,3,5, Florence Margottin-Goguet2,5, Catherine Lacombe1,3,5, Patrick Mayeux1,3,5, and Frédérique Verdier1,3,5 1 Institut Cochin, Département d'Hématologie, Paris, France; 2 Institut Cochin, Département des Maladies Infectieuses, Paris, France; 3 Institut National de la Santé et de la Recherche, Médicale (INSERM), U567, Paris, France; 4 Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 8104, Paris, France; 5 Université Paris Descartes, Faculté de Médecine René Descartes, UMR-S 8104, Paris, France; 6 Laboratoire d'Hématologie, Assistance Publique-Hôpitaux de Paris, Hôpital Cochin, Paris, France Control of intensity and duration of erythropoietin (Epo) signaling is necessary to tightly regulate red blood cell production. We have recently shown that the ubiquitin/proteasome system plays a major role in the control of Epo-R signaling. Indeed, after Epo stimulation, Epo-R is ubiquitinated and its intracellular part is degraded by the proteasome, preventing further signal transduction. The remaining part of the receptor and associated Epo are internalized and degraded by the lysosomes. We show that ß-Trcp is responsible for Epo-R ubiquitination and degradation. After Epo stimulation, ß-Trcp binds to the Epo-R. This binding, like Epo-R ubiquitination, requires Jak2 activation. The Epo-R contains a typical DSG binding sequence for ß-Trcp that is highly conserved among species. Interestingly, this sequence is located in a region of the Epo-R that is deleted in patients with familial polycythemia. Mutation of the serine residue of this motif to alanine (Epo-RS462A) abolished ß-Trcp binding, Epo-R ubiquitination, and degradation. Epo-RS462A activation was prolonged and BaF3 cells expressing this receptor are hypersensitive to Epo, suggesting that part of the hypersensitivity to Epo in familial polycythemia could be the result of the lack of ß-Trcp recruitment to the Epo-R. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Jia, R. Warin, X. Yu, R. Epstein, and C. T. Noguchi Erythropoietin signaling promotes transplanted progenitor cell survival FASEB J, September 1, 2009; 23(9): 3089 - 3099. [Abstract] [Full Text] [PDF] R. Sulahian, O. Cleaver, and L. J.-s. Huang Ligand-induced EpoR internalization is mediated by JAK2 and p85 and is impaired by mutations responsible for primary familial and congenital polycythemia Blood, May 21, 2009; 113(21): 5287 - 5297. [Abstract] [Full Text] [PDF] T. M. Piazza, J.-C. Lu, K. C. Carver, and L. A. 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Tonti Erythropoietin and Its Receptor in Breast Cancer: Putting Together the Pieces of the Puzzle Oncologist, July 1, 2008; 13(7): 761 - 768. [Abstract] [Full Text] [PDF] G. Swaminathan, B. Varghese, C. Thangavel, C. J Carbone, A. Plotnikov, K G S. Kumar, E. M Jablonski, C. V Clevenger, V. Goffin, L. Deng, et al. Prolactin stimulates ubiquitination, initial internalization, and degradation of its receptor via catalytic activation of Janus kinase 2 J. Endocrinol., February 1, 2008; 196(2): R1 - R7. [Abstract] [Full Text] [PDF]

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