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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5223-5229. Prepublished online as a Blood First Edition Paper on March 12, 2007; DOI 10.1182/blood-2006-08-036467. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-08-036467v1 109/12/5223    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fabriek, B. O. Articles by van den Berg, T. K. Search for Related Content PubMed PubMed Citation Articles by Fabriek, B. O. Articles by van den Berg, T. K. Related Collections Hematopoiesis and Stem Cells Phagocytes Cell Adhesion and Motility Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor Babs O. Fabriek1, Machteld M. J. Polfliet1, Rianka P. M. Vloet1, Roel C. van der Schors2, Antoon J. M. Ligtenberg3, Lehn K. Weaver4, Christiaan Geest5, Kenjiro Matsuno6, Søren K. Moestrup7, Christien D. Dijkstra1, and Timo K. van den Berg1,8 1 Department of Molecular Cell Biology, Vrije Universiteit Medical Center, Amsterdam, the Netherlands; 2 Department of Molecular and Cellular Neurobiology, Research Institute Neurosciences, Faculty of Biology, Vrije Universiteit, Amsterdam, the Netherlands; 3 Department of Oral Biochemistry, Academic Center for Dentistry (ACTA), Amsterdam, the Netherlands; 4 Department of Immunology and Microbiology, Dartmouth Medical School, Lebanon, NH; 5 Department of Immunology, University Medical Center Utrecht, the Netherlands; 6 Department of Anatomy, Dokkyo University, School of Medicine, Tochigi, Japan; 7 Department of Medical Biochemistry, University of Aarhus, Denmark; 8 Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Center (AMC), University of Amsterdam, the Netherlands Erythropoiesis occurs in erythroblastic islands, where developing erythroblasts closely interact with macrophages. The adhesion molecules that govern macrophage-erythroblast contact have only been partially defined. Our previous work has implicated the rat ED2 antigen, which is highly expressed on the surface of macrophages in erythroblastic islands, in erythroblast binding. In particular, the monoclonal antibody ED2 was found to inhibit erythroblast binding to bone marrow macrophages. Here, we identify the ED2 antigen as the rat CD163 surface glycoprotein, a member of the group B scavenger receptor cysteine-rich (SRCR) family that has previously been shown to function as a receptor for hemoglobin-haptoglobin (Hb-Hp) complexes and is believed to contribute to the clearance of free hemoglobin. CD163 transfectants and recombinant protein containing the extracellular domain of CD163 supported the adhesion of erythroblastic cells. Furthermore, we identified a 13–amino acid motif (CD163p2) corresponding to a putative interaction site within the second scavenger receptor domain of CD163 that could mediate erythroblast binding. Finally, CD163p2 promoted erythroid expansion in vitro, suggesting that it enhanced erythroid proliferation and/or survival, but did not affect differentiation. These findings identify CD163 on macrophages as an adhesion receptor for erythroblasts in erythroblastic islands, and suggest a regulatory role for CD163 during erythropoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Scavenger receptor helps erythroblasts stay on island Mark J. Koury Blood 2007 109: 5074-5075. [Full Text] [PDF] This article has been cited by other articles: B. O. Fabriek, R. van Bruggen, D. M. Deng, A. J. M. Ligtenberg, K. Nazmi, K. Schornagel, R. P. M. Vloet, C. D. Dijkstra, and T. K. van den Berg The macrophage scavenger receptor CD163 functions as an innate immune sensor for bacteria Blood, January 22, 2009; 113(4): 887 - 892. [Abstract] [Full Text] [PDF] K. A. Kusi, B. A. Gyan, B. Q. Goka, D. Dodoo, G. Obeng-Adjei, M. Troye-Blomberg, B. D. Akanmori, and J. P. Adjimani Levels of Soluble CD163 and Severity of Malaria in Children in Ghana Clin. Vaccine Immunol., September 1, 2008; 15(9): 1456 - 1460. [Abstract] [Full Text] [PDF] J. A. Chasis and N. Mohandas Erythroblastic islands: niches for erythropoiesis Blood, August 1, 2008; 112(3): 470 - 478. [Abstract] [Full Text] [PDF] M. M. Rhodes, P. Kopsombut, M. C. Bondurant, J. O. Price, and M. J. Koury Adherence to macrophages in erythroblastic islands enhances erythroblast proliferation and increases erythrocyte production by a different mechanism than erythropoietin Blood, February 1, 2008; 111(3): 1700 - 1708. [Abstract] [Full Text] [PDF]

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