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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5293-5300. Prepublished online as a Blood First Edition Paper on March 9, 2007; DOI 10.1182/blood-2006-11-058438. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-11-058438v1 109/12/5293    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sundstrom, J. B. Articles by Ansari, A. A. Search for Related Content PubMed PubMed Citation Articles by Sundstrom, J. B. Articles by Ansari, A. A. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Human tissue mast cells are an inducible reservoir of persistent HIV infection J. Bruce Sundstrom1, Jane E. Ellis2, Gregory A. Hair1, Arnold S. Kirshenbaum3, Dean D. Metcalfe3, Hong Yi4, Adriana C. Cardona1, Michael K. Lindsay2, and Aftab A. Ansari1 1 Department of Pathology and Laboratory Medicine and 2 Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA; 3 Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD; 4 School of Medicine Microscopy Core, Emory University School of Medicine, Atlanta, GA We have proposed that, unlike other HIV-vulnerable cell lineages, progenitor mast cells (prMCs), cultured in vitro from undifferentiated bone marrow–derived CD34+ pluripotent progenitors (PPPs), are susceptible to infection during a limited period of their ontogeny. As infected prMCs mature in culture, they lose expression of viral chemokine coreceptors necessary for viral entry and develop into long-lived, latently infected mature tissue mast cells (MCs), resistant to new infection. In vivo recruitment of prMCs to different tissue compartments occurs in response to tissue injury, growth, and remodeling or allergic inflammation, allowing populations of circulating and potentially HIV-susceptible prMCs to spread persistent infection to diverse tissue compartments. In this report, we provide in vivo evidence to confirm this model by demonstrating that HIV-infected women have both circulating prMCs and placental tissue MCs (PLMCs) that harbor inducible infectious HIV even after highly active antiretroviral therapy (HAART) during pregnancy. Furthermore, infectious virus, capable of infecting alloactivated fetal cord blood mononuclear cells (CBMCs), could be induced in isolated latently infected PLMCs after weeks in culture in vitro. These data provide the first in vivo evidence that tissue MCs, developed from infected circulating prMCs, comprise a long-lived inducible reservoir of persistent HIV in infected persons during HAART. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. B. Dinoso, S. A. Rabi, J. N. Blankson, L. Gama, J. L. Mankowski, R. F. Siliciano, M. C. Zink, and J. E. Clements A Simian Immunodeficiency Virus-Infected Macaque Model To Study Viral Reservoirs That Persist during Highly Active Antiretroviral Therapy J. Virol., September 15, 2009; 83(18): 9247 - 9257. [Abstract] [Full Text] [PDF] T. P. Brennan, J. O. Woods, A. R. Sedaghat, J. D. Siliciano, R. F. Siliciano, and C. O. Wilke Analysis of Human Immunodeficiency Virus Type 1 Viremia and Provirus in Resting CD4+ T Cells Reveals a Novel Source of Residual Viremia in Patients on Antiretroviral Therapy J. Virol., September 1, 2009; 83(17): 8470 - 8481. [Abstract] [Full Text] [PDF] J. B. Sundstrom, G. A. Hair, A. A. Ansari, W. E. Secor, A. M. Gilfillan, D. D. Metcalfe, and A. S. Kirshenbaum IgE-Fc{epsilon}RI Interactions Determine HIV Coreceptor Usage and Susceptibility to Infection during Ontogeny of Mast Cells J. Immunol., May 15, 2009; 182(10): 6401 - 6409. [Abstract] [Full Text] [PDF]

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