SEARCH:   Advanced

Blood, 15 June 2007, Vol. 109, No. 12, pp. 5371-5379. Prepublished online as a Blood First Edition Paper on March 1, 2007; DOI 10.1182/blood-2006-08-038422. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-08-038422v1 109/12/5371    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Piccioli, D. Articles by Wack, A. Search for Related Content PubMed PubMed Citation Articles by Piccioli, D. Articles by Wack, A. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets Diego Piccioli1, Simona Tavarini1, Erica Borgogni1, Veronica Steri1, Sandra Nuti1, Chiara Sammicheli1, Monia Bardelli2, Daniela Montagna3, Franco Locatelli3, and Andreas Wack1 1 Molecular Immunology Department, Research Center, Novartis Vaccines, Siena, Italy; 2 Molecular Biology Department, Università degli Studi, Siena, Italy; 3 Department of Pediatrics, University of Pavia, Pediatric Hematology Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Matteo, Pavia, Italy Human blood contains 2 populations of dendritic cells (DCs): plasmacytoid and myeloid (mDC). mDCs are subdivided into 3 subsets using the surface markers CD16, CD1c, and BDCA-3. Their role as pathogen sentinels and adjuvant targets was tested by phenotypic and functional analysis. We show that mDC subsets are immature and express mRNA for most toll-like receptors (TLRs), except for TLR3 in CD16-mDCs. The most represented subsets, CD16- and CD1c-mDCs, are similarly responsive to all TLR agonists. Among 31 cytokines tested, both subsets produce CXCL8 (IL-8)/tumor necrosis factor- (TNF-)/IL-6/CCL3 (MIP-1)/CCL4 (MIP-1ß)/IL-1ß. CXCL8 (IL-8) is the predominant cytokine produced by CD1c-mDCs on TLR engagement, whereas all other cytokines, particularly TNF-, are secreted in 10-fold to 100-fold higher amounts by CD16-mDCs. CD16-mDCs cocultured with human umbilical vein endothelial cells induce a significantly higher production of CXCL10 (IP-10), granulocyte-macrophage colony-stimulating factor, and granulocyte colony-stimulating factor than CD1c-mDCs. In addition, interleukin-3 and type I interferons are stimuli specifically for DC maturation rather than cytokine secretion, whereas TNF- is almost ineffective in inducing either function, suggesting a mechanism of T-cell–DC crosstalk and of rapid induction of antigen-presenting cell function during viral infection rather than inflammation. In conclusion, CD16-mDCs show strong proinflammatory activity, whereas CD1c-mDCs appear to be mainly inducers of chemotaxis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Huysamen, J. A. Willment, K. M. Dennehy, and G. D. Brown CLEC9A Is a Novel Activation C-type Lectin-like Receptor Expressed on BDCA3+ Dendritic Cells and a Subset of Monocytes J. Biol. Chem., June 13, 2008; 283(24): 16693 - 16701. [Abstract] [Full Text] [PDF]

	Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020