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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5511-5519. Prepublished online as a Blood First Edition Paper on March 5, 2007; DOI 10.1182/blood-2007-01-069757. This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: blood-2007-01-069757v1 109/12/5511    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Weissinger, E. M. Articles by Ganser, A. Search for Related Content PubMed PubMed Citation Articles by Weissinger, E. M. Articles by Ganser, A. Related Collections Immunobiology Transplantation Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Proteomic patterns predict acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation Eva M. Weissinger1, Eric Schiffer2, Bernd Hertenstein1,3, James L. Ferrara4, Ernst Holler5, Michael Stadler1, Hans-Jochem Kolb6, Axel Zander7, Petra Zürbig2, Markus Kellmann8, and Arnold Ganser1 1 Hannover Medical School, Department of Hematology, Hemostasis, and Oncology, Hannover, Germany; 2 Mosaiques-Diagnostics and Therapeutics AG, Hannover Germany; 3 Klinikum Bremen Mitte GmbH, Innere Medizin I, Bremen, Germany; 4 Department of Pediatrics and Internal Medicine, University of Michigan Medical School, Ann Arbor, MI; 5 University of Regensburg, Department of Hematology, Bone Marrow Transplantation Unit, Regensburg, Germany; 6 Clinical Cooperative Group for Hematopoietic Stem Cell Transplantation, Department of Medicine III, Ludwig-Maximilians-University, Munich, Germany; 7 University Clinic Hamburg-Eppendorf, Bone Marrow Transplant Unit, Hamburg, Germany; 8 Thermo Electron, Bremen, Germany Acute graft-versus-host disease (aGvHD) contributes significantly to morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Diagnosis of GvHD is mainly based on clinical features and tissue biopsies. A noninvasive, unbiased laboratory test for GvHD diagnosis does not exist. Here we describe the application of capillary electrophoresis coupled online with mass spectrometry (CE-MS) to 13 samples from 10 patients with aGvHD of grade II or more and 50 control samples from 23 patients without GvHD. About 170 GvHD-specific polypeptides were detected and a tentatively aGvHD-specific model consisting of 31 polypeptides was chosen, allowing correct classification of 13 of 13 (sensitivity 100.0% [95% confidence interval {CI} 75.1 to 100.0]) aGvHD samples and 49 of 50 (specificity 98.0% [95% CI 89.3 to 99.7]) control samples of the training set. The subsequent blinded evaluation of 599 samples enabled diagnosis of aGvHD greater than grade II, even prior to clinical diagnosis, with a sensitivity of 83.1% (95% CI 73.1 to 87.9) and a specificity of 75.6% (95% CI 71.6 to 79.4). Thus, high-resolution proteome analysis represents an unbiased laboratory-based screening method, enabling diagnosis, and possibly enabling preemptive therapy. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Clinical proteomics: towards diagnostics and prognostics Visith Thongboonkerd Blood 2007 109: 5075-5076. [Full Text] [PDF] This article has been cited by other articles: K. Bramham, H.D. Mistry, L. Poston, L.C. Chappell, and A.J. Thompson The non-invasive biopsy--will urinary proteomics make the renal tissue biopsy redundant? QJM, June 24, 2009; (2009) hcp071v1. [Abstract] [Full Text] [PDF] G. Socie Graft-versus-host disease: proteomics comes of age Blood, January 8, 2009; 113(2): 271 - 272. [Full Text] [PDF] S. Paczesny, O. I. Krijanovski, T. M. Braun, S. W. Choi, S. G. Clouthier, R. Kuick, D. E. Misek, K. R. Cooke, C. L. Kitko, A. Weyand, et al. A biomarker panel for acute graft-versus-host disease Blood, January 8, 2009; 113(2): 273 - 278. [Abstract] [Full Text] [PDF] H. Baharvand, A. Fathi, D. van Hoof, and G. H. Salekdeh Concise Review: Trends in Stem Cell Proteomics Stem Cells, August 1, 2007; 25(8): 1888 - 1903. [Abstract] [Full Text] [PDF]

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