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Blood, 15 January 2007, Vol. 109, No. 2, pp. 543-545. Prepublished online as a Blood First Edition Paper on September 21, 2006; DOI 10.1182/blood-2006-06-030270. This Article Full Text Full Text (PDF) Supplemental Table All Versions of this Article: blood-2006-06-030270v1 109/2/543    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by McKenzie, J. L. Articles by Dick, J. E. Search for Related Content PubMed PubMed Citation Articles by McKenzie, J. L. Articles by Dick, J. E. Related Collections Hematopoiesis and Stem Cells Stem Cells in Hematology Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Brief report Low rhodamine 123 retention identifies long-term human hematopoietic stem cells within the Lin–CD34+CD38– population Joby L. McKenzie1,2, Katsuto Takenaka1, Olga I. Gan1, Monica Doedens1, and John E. Dick1,2, 1 Division of Cell and Molecular Biology, University Health Network and 2 Department of Molecular and Medical Genetics, University of Toronto, ON, Canada Progress to uncover the molecular and cellular regulators that govern human hematopoietic stem cell (HSC) fate has been impeded by an inability to obtain highly purified fractions of HSCs. We report that the rhodamine 123 (Rho 123) dye effluxing fraction of the Lin–CD34+CD38– population contains SCID-repopulating cells (SRCs) capable of long-term repopulation in primary nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Purification based on Rho uptake led to a 4-fold enrichment of SRCs in the Lin–CD34+CD38– fraction, with a frequency of 1 SRC in 30 Lin–CD34+CD38–Rholo cells. The Lin–CD34+CD38–Rholo fraction also possesses long-term self-renewal capacity as measured by serial transplantation totaling more than 20 weeks. We conclude that Rho dye efflux provides an additional means of purifying human HSCs in the quest to achieve homogeneous populations of primitive cells for both experimental and therapeutic applications. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Hills, K. Lucke, E. A. Chavez, C. J. Eaves, and P. M. Lansdorp Probing the mitotic history and developmental stage of hematopoietic cells using single telomere length analysis (STELA) Blood, June 4, 2009; 113(23): 5765 - 5775. [Abstract] [Full Text] [PDF] U. Blank, G. Karlsson, and S. Karlsson Signaling pathways governing stem-cell fate Blood, January 15, 2008; 111(2): 492 - 503. [Abstract] [Full Text] [PDF] O. Christ, K. Lucke, S. Imren, K. Leung, M. Hamilton, A. Eaves, C. Smith, and C. Eaves Improved purification of hematopoietic stem cells based on their elevated aldehyde dehydrogenase activity Haematologica, September 1, 2007; 92(9): 1165 - 1172. [Abstract] [Full Text] [PDF]

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