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Blood, 15 January 2007, Vol. 109, No. 2, pp. 626-631. Prepublished online as a Blood First Edition Paper on September 22, 2006; DOI 10.1182/blood-2006-02-003665. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-02-003665v1 blood-2006-02-003665v2 109/2/626    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Del Prete, A. Articles by Haribabu, B. Search for Related Content PubMed PubMed Citation Articles by Del Prete, A. Articles by Haribabu, B. Related Collections Immunobiology Cell Adhesion and Motility Signal Transduction Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Regulation of dendritic cell migration and adaptive immune response by leukotriene B4 receptors: a role for LTB4 in up-regulation of CCR7 expression and function Annalisa Del Prete1,2, Wen-Hai Shao1, Stefania Mitola3, Giuseppe Santoro2, Silvano Sozzani3, and Bodduluri Haribabu1, 1 Tumor Immunobiology Program, James Graham Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville Health Sciences Center, KY; 2 Section of Clinical Biochemistry, Department of Medical Biochemistry, University of Bari, Italy; 3 Section of General Pathology and Immunology, University of Brescia, Brescia, and Istituto Clinico Humanitas, Rozzano, Italy Trafficking of dendritic cells (DCs) to peripheral tissues and to secondary lymphoid organs depends on chemokines and lipid mediators. Here, we show that bone marrow–derived DCs (BM-DCs) express functional leukotriene B4 (LTB4) receptors as observed in dose-dependent chemotaxis and calcium mobilization responses. LTB4, at low concentrations, promoted the migration of immature and mature DCs to CCL19 and CCL21, which was associated with a rapid (30-minute) increase of CCR7 expression at the membrane level. At longer incubation times (6 hours), gene array analysis revealed a promoting role of LTB4, showing a significant increase of CCR7 and CCL19 mRNA levels. BM-DCs cultured from BLT1–/– or BLT1/2–/– mice showed a normal phenotype, but in vivo BLT1/2–/–DCs showed dramatic decrease in migration to the draining lymph nodes relative to wild-type (WT) DCs. Consistent with these observations, BLT1/2–/– mice showed a reduced response in a model of 2,4-dinitro-fluorobenzene (DNFB)–induced contact hypersensitivity. Adoptive transfer of 2,4-dinitrobenzene sulfonic acid (DNBS)–pulsed DCs directly implicated the defect in DC migration to lymph node with the defect in contact hypersensitivity. These results provide strong evidence for a role of LTB4 in regulating DC migration and the induction of adaptive immune responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. K. Vassiliou, O. M. Kesler, J. H. Tadros, and D. Ganea Bone Marrow-Derived Dendritic Cells Generated in the Presence of Resolvin E1 Induce Apoptosis of Activated CD4+ T Cells J. Immunol., October 1, 2008; 181(7): 4534 - 4544. [Abstract] [Full Text] [PDF] R. van de Ven, G. L. Scheffer, A. W. Reurs, J. J. Lindenberg, R. Oerlemans, G. Jansen, J.-P. Gillet, J. N. Glasgow, A. Pereboev, D. T. Curiel, et al. A role for multidrug resistance protein 4 (MRP4; ABCC4) in human dendritic cell migration Blood, September 15, 2008; 112(6): 2353 - 2359. [Abstract] [Full Text] [PDF] N. Miyahara, H. Ohnishi, H. Matsuda, S. Miyahara, K. Takeda, T. Koya, S. Matsubara, M. Okamoto, A. Dakhama, B. Haribabu, et al. Leukotriene B4 Receptor 1 Expression on Dendritic Cells Is Required for the Development of Th2 Responses and Allergen-Induced Airway Hyperresponsiveness J. Immunol., July 15, 2008; 181(2): 1170 - 1178. [Abstract] [Full Text] [PDF] I. Valera, N. Fernandez, A. G. Trinidad, S. Alonso, G. D. Brown, A. Alonso, and M. S. Crespo Costimulation of Dectin-1 and DC-SIGN Triggers the Arachidonic Acid Cascade in Human Monocyte-Derived Dendritic Cells J. Immunol., April 15, 2008; 180(8): 5727 - 5736. [Abstract] [Full Text] [PDF] K. Kabashima, N. Shiraishi, K. Sugita, T. Mori, A. Onoue, M. Kobayashi, J.-i. Sakabe, R. Yoshiki, H. Tamamura, N. Fujii, et al. CXCL12-CXCR4 Engagement Is Required for Migration of Cutaneous Dendritic Cells Am. J. Pathol., October 1, 2007; 171(4): 1249 - 1257. [Abstract] [Full Text] [PDF] A. L. Christy and M. A. Brown The Multitasking Mast Cell: Positive and Negative Roles in the Progression of Autoimmunity J. Immunol., September 1, 2007; 179(5): 2673 - 2679. [Abstract] [Full Text] [PDF]

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