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Blood, 1 February 2007, Vol. 109, No. 3, pp. 1034-1042.
Prepublished online as a Blood First Edition Paper on October 5, 2006; DOI 10.1182/blood-2006-06-027912.


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IMMUNOBIOLOGY

IL-7–induced proliferation of recent thymic emigrants requires activation of the PI3K pathway

Louise Swainson1,2, Sandrina Kinet1,2, Cedric Mongellaz1,2, Marion Sourisseau1,2, Telmo Henriques1,2, and Naomi Taylor1,2

1 Institut de Génétique Moléculaire de Montpellier, Montpellier, France; 2 Centre National de la Recherche Scientifique (CNRS), Université de Montpellier II (UMR), Montpellier, France

The IL-7 cytokine promotes the survival of a diverse T-cell pool, thereby ensuring an efficient immune response. Moreover, IL-7 induces the proliferation of recent thymic emigrants (RTEs) in neonates. Here, we demonstrate that the survival and proliferative effects of IL-7 on human RTEs can be distinguished on the basis of dose as well as duration of IL-7 administration. A dose of 0.1 ng/mL IL-7 is sufficient to promote viability, whereas cell-cycle entry is observed only at doses higher than 1 ng/mL. Moreover, a short 1-hour exposure to high-dose IL-7 (10 ng/mL) induces long-term survival but continuous IL-7 exposure is necessary for optimal cell-cycle entry and proliferation. We find that distinct signaling intermediates are activated under conditions of IL-7–induced survival and proliferation; STAT5 tyrosine phosphorylation does not correlate with proliferation, whereas up-regulation of the glucose transporter Glut-1 as well as increased glucose uptake are markers of IL-7–induced cell cycle entry. Glut-1 is directly regulated by PI3K and, indeed, inhibiting PI3K activity abrogates IL-7–induced proliferation. Our finding that the survival and proliferation of RTEs are differentially modulated by the dose and kinetics of exogenous IL-7 has important implications for the clinical use of this cytokine.


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