|
|
Blood, 1 February 2007, Vol. 109, No. 3, pp. 1298-1306.
Prepublished online as a Blood First Edition Paper on September 26, 2006; DOI 10.1182/blood-2006-06-030551.
 Previous Article | Table of Contents | Next Article 
STEM CELLS IN HEMATOLOGY
Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment
Fernando Anjos-Afonso1, and
Dominique Bonnet1
1 Haematopoietic Stem Cell Lab, Cancer Research UK, London Research Institute, London, United Kingdom
It is believed that a primitive cell type that maintains the mesenchymal compartment exists in the bone marrow. However, this putative mesenchymal stem/progenitor cell is yet to be identified and isolated. We are reporting the identification, isolation, and detailed characterization of the most primitive mesenchymal progenitor cells in the adult murine bone marrow, based on the expression of stage-specific embryonic antigen–1 (SSEA-1). This primitive subset can be identified in mesenchymal cell cultures and also directly in the bone marrow, thus ascertaining for the first time their existence in an adult organism. Characterization of SSEA-1+ mesenchymal cells revealed that upon purification these cells gave rise to SSEA-1– mesenchymal cells, whereas the reverse could not be observed. Also, these SSEA-1+ cells have a much higher capacity to differentiate than their negative counterparts, not only to several mesenchymal cell types but also to unconventional cell types such as astrocyte-, endothelial-, and hepatocyte-like cells in vitro. Most importantly, a single-cell–derived population was capable of differentiating abundantly into different mesenchymal cell types in vivo. Altogether we are proposing a hierarchical organization of the mesenchymal compartment, placing SSEA-1+ cells at the apex of this hierarchy.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. Delorme, J. Ringe, C. Pontikoglou, J. Gaillard, A. Langonne, L. Sensebe, D. Noel, C. Jorgensen, T. Haupl, and P. Charbord
Specific Lineage-Priming of Bone Marrow Mesenchymal Stem Cells Provides the Molecular Framework for Their Plasticity
Stem Cells,
May 1, 2009;
27(5):
1142 - 1151.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Haniffa, M. P. Collin, C. D. Buckley, and F. Dazzi
Mesenchymal stem cells: the fibroblasts' new clothes?
Haematologica,
February 1, 2009;
94(2):
258 - 263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. H. Lee, M. J. Seo, A. A. Pulin, C. A. Gregory, J. Ylostalo, and D. J. Prockop
The CD34-like protein PODXL and {alpha}6-integrin (CD49f) identify early progenitor MSCs with increased clonogenicity and migration to infarcted heart in mice
Blood,
January 22, 2009;
113(4):
816 - 826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Delorme, J. Ringe, N. Gallay, Y. Le Vern, D. Kerboeuf, C. Jorgensen, P. Rosset, L. Sensebe, P. Layrolle, T. Haupl, et al.
Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells
Blood,
March 1, 2008;
111(5):
2631 - 2635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Nauta and W. E. Fibbe
Immunomodulatory properties of mesenchymal stromal cells
Blood,
November 15, 2007;
110(10):
3499 - 3506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Phinney and D. J. Prockop
Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair Current Views
Stem Cells,
November 1, 2007;
25(11):
2896 - 2902.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
