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Blood, 1 February 2007, Vol. 109, No. 3, pp. 1298-1306. Prepublished online as a Blood First Edition Paper on September 26, 2006; DOI 10.1182/blood-2006-06-030551. This Article Full Text Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2006-06-030551v1 109/3/1298    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Anjos-Afonso, F. Articles by Bonnet, D. Search for Related Content PubMed PubMed Citation Articles by Anjos-Afonso, F. Articles by Bonnet, D. Related Collections Stem Cells in Hematology Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  STEM CELLS IN HEMATOLOGY Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment Fernando Anjos-Afonso1, and Dominique Bonnet1 1 Haematopoietic Stem Cell Lab, Cancer Research UK, London Research Institute, London, United Kingdom It is believed that a primitive cell type that maintains the mesenchymal compartment exists in the bone marrow. However, this putative mesenchymal stem/progenitor cell is yet to be identified and isolated. We are reporting the identification, isolation, and detailed characterization of the most primitive mesenchymal progenitor cells in the adult murine bone marrow, based on the expression of stage-specific embryonic antigen–1 (SSEA-1). This primitive subset can be identified in mesenchymal cell cultures and also directly in the bone marrow, thus ascertaining for the first time their existence in an adult organism. Characterization of SSEA-1+ mesenchymal cells revealed that upon purification these cells gave rise to SSEA-1– mesenchymal cells, whereas the reverse could not be observed. Also, these SSEA-1+ cells have a much higher capacity to differentiate than their negative counterparts, not only to several mesenchymal cell types but also to unconventional cell types such as astrocyte-, endothelial-, and hepatocyte-like cells in vitro. Most importantly, a single-cell–derived population was capable of differentiating abundantly into different mesenchymal cell types in vivo. Altogether we are proposing a hierarchical organization of the mesenchymal compartment, placing SSEA-1+ cells at the apex of this hierarchy. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Muneoka The Biology of Regenerative Medicine Sci. Signal., September 2, 2008; 1(35): pe30 - pe30. [Abstract] [Full Text] [PDF] B. Delorme, J. Ringe, N. Gallay, Y. Le Vern, D. Kerboeuf, C. Jorgensen, P. Rosset, L. Sensebe, P. Layrolle, T. Haupl, et al. Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells Blood, March 1, 2008; 111(5): 2631 - 2635. [Abstract] [Full Text] [PDF] A. J. Nauta and W. E. Fibbe Immunomodulatory properties of mesenchymal stromal cells Blood, November 15, 2007; 110(10): 3499 - 3506. [Abstract] [Full Text] [PDF] D. G. Phinney and D. J. Prockop Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair Current Views Stem Cells, November 1, 2007; 25(11): 2896 - 2902. [Abstract] [Full Text] [PDF] K. Muneoka The Biology of Regenerative Medicine Sci. Signal., June 5, 2007; 2007(389): pe30 - pe30. [Abstract] [Full Text] [PDF]

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