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Blood, 1 February 2007, Vol. 109, No. 3, pp. 995-1002. Prepublished online as a Blood First Edition Paper on September 26, 2006; DOI 10.1182/blood-2006-02-004945. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-02-004945v1 109/3/995    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Moosbauer, C. Articles by Engelmann, B. Search for Related Content PubMed PubMed Citation Articles by Moosbauer, C. Articles by Engelmann, B. Related Collections Hemostasis, Thrombosis, and Vascular Biology Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Eosinophils are a major intravascular location for tissue factor storage and exposure Christine Moosbauer1, Eberhard Morgenstern2, Susan L. Cuvelier3, Davit Manukyan1, Kiril Bidzhekov1, Sybille Albrecht4, Peter Lohse1, Kamala D. Patel3, and Bernd Engelmann1 1 Vaskuläre Biologie und Hämostase, Institut für Klinische Chemie, Ludwig-Maximilians-Universität, Munich, Germany; 2 Anatomie and Zellbiologie, Universität des Saarlandes, Homburg, Germany; 3 Department of Physiology and Biophysics, Immunology Research Group, University of Calgary, AB, Canada; and 4 Institut für Pathologie, Technische Universität Dresden, Germany Blood cell progenitors were scanned for the presence of the coagulation starter protein tissue factor (TF) by immunoelectron microscopy. Thereby, substantial TF expression was observed in the precursor cells of eosinophils. TF levels were lower in basophil precursors and barely detectable in neutrophil progenitors. In peripheral blood immediately processed to avoid activation of the TF gene, mature eosinophils were found to considerably express TF, unique among the granulocyte and monocyte fractions. TF was preferentially located in the specific granules in resting eosinophils. Platelet-activating factor (PAF), and more pronounced, granulocyte-macrophage colony-stimulating factor (GM-CSF) plus PAF, caused translocation of preformed TF to the eosinophil cell membrane. GM-CSF/PAF also increased the TF transcript levels. The activated eosinophils exhibited procoagulant activity that was abrogated by TF inhibition. Targeting the extracellular domain of TF with specific antibodies markedly suppressed the initial phase of the eosinophil passage across the IL-4–activated endothelium. Eosinophil rolling and firm adhesion remained unaffected. This suggests that TF specifically facilitates the early transendothelial migration of the eosinophils. In summary, eosinophils maintain a high TF expression during maturation, providing a main source of preformed TF in blood, which might be relevant for the thrombogenesis promoted by hypereosinophilic conditions. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Shinagawa, V. A. Ploplis, and F. J. Castellino A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice Am J Physiol Lung Cell Mol Physiol, May 1, 2009; 296(5): L763 - L770. [Abstract] [Full Text] [PDF] E. M. Egorina, M. A. Sovershaev, J. O. Olsen, and B. Osterud Granulocytes do not express but acquire monocyte-derived tissue factor in whole blood: evidence for a direct transfer Blood, February 1, 2008; 111(3): 1208 - 1216. [Abstract] [Full Text] [PDF] N. Mackman, R. E. Tilley, and N. S. Key Role of the Extrinsic Pathway of Blood Coagulation in Hemostasis and Thrombosis Arterioscler. Thromb. Vasc. Biol., August 1, 2007; 27(8): 1687 - 1693. [Abstract] [Full Text] [PDF]

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