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Blood, 1 March 2007, Vol. 109, No. 5, pp. 1825-1833. Prepublished online as a Blood First Edition Paper on October 31, 2006; DOI 10.1182/blood-2006-05-023028. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-05-023028v1 109/5/1825    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Forde, S. Articles by Watt, S. M. Search for Related Content PubMed PubMed Citation Articles by Forde, S. Articles by Watt, S. M. Related Collections Hematopoiesis and Stem Cells Transplantation Cell Adhesion and Motility Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES, CYTOKINES, AND INTERLEUKINS Endolyn (CD164) modulates the CXCL12-mediated migration of umbilical cord blood CD133+ cells Sinead Forde1,2, Britt Jorgensen Tye1,2, Sarah E. Newey1, Maria Roubelakis1,2, Jon Smythe1, Colin P. McGuckin3, Ruth Pettengell4, and Suzanne M. Watt1,2 1 Stem Cell Laboratory, National Blood Service, National Blood and Transplant Authority, Oxford, United Kingdom; 2 Nuffield Department of Clinical Laboratory Sciences, University of Oxford, United Kingdom; 3 Haematological Sciences and the International Centre for Life, University of Newcastle upon Tyne, United Kingdom; 4 St George's, University of London, United Kingdom Hematopoietic stem cell/hematopoietic progenitor cell (HSC/HPC) homing to specific microenvironmental niches involves interactions between multiple receptor ligand pairs. Although CXCL12/CXCR4 plays a central role in these events, CXCR4 regulators that provide the specificity for such cells to lodge and be retained in particular niches are poorly defined. Here, we provide evidence that the sialomucin endolyn (CD164), an adhesion receptor that regulates the adhesion of CD34+ cells to bone marrow stroma and the recruitment of CD34+CD38lo/– cells into cycle, associates with CXCR4. The class II 103B2 monoclonal antibody, which binds the CD164 N-linked glycan-dependent epitope or CD164 knockdown by RNA interference, significantly inhibits the migration of CD133+ HPCs toward CXCL12 in vitro. On presentation of CXCL12 on fibronectin, CD164 associates with CXCR4, an interaction that temporally follows the association of CXCR4 with the integrins VLA-4 and VLA-5. This coincides with PKC- and Akt signaling through the CXCR4 receptor, which was disrupted on the loss of CD164 though MAPK signaling was unaffected. We therefore demonstrate a novel association among 3 distinct families of cell-surface receptors that regulate cell migratory responses and identify a new role for CD164. We propose that this lends specificity to the homing and lodgment of these cells within the bone marrow niche. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G.-U. Bae, U. Gaio, Y.-J. Yang, H.-J. Lee, J.-S. Kang, and R. S. Krauss Regulation of Myoblast Motility and Fusion by the CXCR4-associated Sialomucin, CD164 J. Biol. Chem., March 28, 2008; 283(13): 8301 - 8309. [Abstract] [Full Text] [PDF]

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