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Blood, 1 March 2007, Vol. 109, No. 5, pp. 1841-1849. Prepublished online as a Blood First Edition Paper on October 26, 2006; DOI 10.1182/blood-2006-02-001578. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-02-001578v1 109/5/1841    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fakruddin, J. M. Articles by Imamichi, T. Search for Related Content PubMed PubMed Citation Articles by Fakruddin, J. M. Articles by Imamichi, T. Related Collections Chemokines, Cytokines, and Interleukins Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CHEMOKINES, CYTOKINES, AND INTERLEUKINS Noninfectious papilloma virus–like particles inhibit HIV-1 replication: implications for immune control of HIV-1 infection by IL-27 J. Mohamad Fakruddin1, Richard A. Lempicki2, Robert J. Gorelick3, Jun Yang2, Joseph W. Adelsberger4, Alfonso J. Garcia-Pineres5, Ligia A. Pinto5, H. Clifford Lane6, and Tomozumi Imamichi1 1 Laboratory of Human Retrovirology, Clinical Services Program (CSP), Science Applications International Corporation (SAIC)–Frederick, National Cancer Institute (NCI)–Frederick, MD; 2 Laboratory of Immunopathogenesis and Bioinformatics, CSP, SAIC-Frederick, NCI-Frederick, MD; 3 Retroviral Mutagenesis Section, AIDS Vaccine Program, SAIC-Frederick, NCI-Frederick, MD; 4 AIDS Monitoring Laboratory, CSP, SAIC-Frederick, NCI-Frederick, MD; 5 HPV Monitoring Laboratory, CSP, SAIC-Frederick, NCI-Frederick, MD; and 6 Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD Human papilloma virus (HPV)–like particles (VLPs) have been used as a vaccine to prevent HPV infection. Recent studies demonstrate that VLPs bind to dendritic cells and induce the expression of antiviral cytokines such as interferon- (IFN-), interleukin-10 (IL-10) and IFN-. In the present study, we evaluated the effect of VLPs on HIV-1 replication in peripheral blood mononuclear cells (PBMCs), CD4+ T cells, and macrophages. Here, we show that VLPs suppress the replication of both X4 and R5 HIV-1 without affecting the expression of CD4, CXCR4, and CCR5. Soluble factor(s) released by PBMCs and macrophages on VLPs treatment inhibited HIV-1 replication. To determine the inhibitory factors, DNA microarray analysis was performed using VLP-treated PBMCs and macrophages. VLPs induced the genes associated with IFN induction, immune responses, and antiviral responses, among with the recently described cytokine IL-27. Subsequently, IL-27 was found to be a potent inhibitor of HIV-1 replication in PBMCs, CD4+ T cells, and macrophages. Taken together, our studies identify a novel role of IL-27 in restricting HIV-1 replication and suggest that further examination of the inhibitory property of IL-27 may pave the way for a novel therapy for HIV-1 infection. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Pirhonen, J. Siren, I. Julkunen, and S. Matikainen IFN-{alpha} regulates Toll-like receptor-mediated IL-27 gene expression in human macrophages J. Leukoc. Biol., November 1, 2007; 82(5): 1185 - 1192. [Abstract] [Full Text] [PDF]

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