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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2514-2520. Prepublished online as a Blood First Edition Paper on November 16, 2006; DOI 10.1182/blood-2006-07-036715. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-07-036715v1 109/6/2514    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Takamatsu, H. Articles by Nakao, S. Search for Related Content PubMed PubMed Citation Articles by Takamatsu, H. Articles by Nakao, S. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Red Cells Cytoskeleton Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Specific antibodies to moesin, a membrane-cytoskeleton linker protein, are frequently detected in patients with acquired aplastic anemia Hiroyuki Takamatsu1, Xingmin Feng1, Tatsuya Chuhjo3, Xuzhang Lu1, Chiharu Sugimori1, Katsuya Okawa4, Miyuki Yamamoto2, Shoichi Iseki2, and Shinji Nakao1 1 Cellular Transplantation Biology, Division of Cancer Medicine, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan; 2 Department of Histology and Embryology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan; 3 Internal Medicine, NTT West Kanazawa Hospital, Kanazawa, Japan; 4 Biomolecular Characterization Unit, Frontier Technology Center HMRO, Kyoto University Graduate School of Medicine, Kyoto, Japan To identify novel autoantibodies in acquired aplastic anemia (AA), we screened the sera of patients with AA possessing small populations of paroxysmal nocturnal hemoglobinuria (PNH)–type cells for the presence of antibodies (Abs) which recognize proteins derived from a leukemia cell line, UT-7. Immunoblotting using proteins derived from lysates or culture supernatants of UT-7 cells revealed the presence of IgG Abs specific to an 80-kDa protein. Peptide mass fingerprinting identified this 80-kDa protein as moesin. Enzyme-linked immunosorbent assay (ELISA) using recombinant moesin showed high titers of antimoesin Abs in 25 (37%) of 67 patients with AA. Moesin was secreted from several myeloid leukemia cell lines other than UT-7, such as OUN-1 and K562, as an exosomal protein. The presence of antimoesin Abs was significantly correlated with the presence of PNH-type cells and antidiazepam-binding inhibitor-related protein-1 (DRS-1) Abs. Patients with AA that did not show any of these 3 markers tended to respond poorly to immunosuppressive therapy. These findings suggest that a B-cell response to moesin, possibly derived from hematopoietic cells, frequently occurs in patients with AA and that detection of antimoesin Abs in combination with other markers may be useful in diagnosing immune pathophysiology in patients with AA. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. L. Espinoza, H. Takamatsu, X. Lu, Z. Qi, and S. Nakao Anti-moesin antibodies derived from patients with aplastic anemia stimulate monocytic cells to secrete TNF-{alpha} through an ERK1/2-dependent pathway Int. Immunol., August 1, 2009; 21(8): 913 - 923. [Abstract] [Full Text] [PDF] H. Takamatsu, J. L. Espinoza, X. Lu, Z. Qi, K. Okawa, and S. Nakao Anti-Moesin Antibodies in the Serum of Patients with Aplastic Anemia Stimulate Peripheral Blood Mononuclear Cells to Secrete TNF-{alpha} and IFN-{gamma} J. Immunol., January 1, 2009; 182(1): 703 - 710. [Abstract] [Full Text] [PDF]

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