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Blood, 1 April 2007, Vol. 109, No. 7, pp. 2806-2814. Prepublished online as a Blood First Edition Paper on December 19, 2006; DOI 10.1182/blood-2006-06-030213. This Article Full Text Full Text (PDF) Supplemental Table and Figure All Versions of this Article: blood-2006-06-030213v1 109/7/2806    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Coupel, S. Articles by Charreau, B. Search for Related Content PubMed PubMed Citation Articles by Coupel, S. Articles by Charreau, B. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation Stéphanie Coupel1, Anne Moreau2, Mohamed Hamidou3, Vaclav Horejsi4, Jean-Paul Soulillou1, and Béatrice Charreau1 1 Institut National de la Santé et de la Recherche Médicale, Unit 643, Institut de Transplantation et de Recherche en Transplantation, Nantes, France; 2 Service d'Anatomo-Pathologie, Centre Hospitalo-Universitaire (CHU) Hôtel-Dieu, Nantes, France; 3 Service de Médecine Interne, CHU Hôtel-Dieu, Nantes, France; 4 Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic Human leukocyte antigen (HLA)–E belongs, with HLA-G and HLA-F, to the nonclassic major histocompatibility complex (MHC) class I (Ib) molecules, broadly defined by a limited polymorphism and a restricted pattern of cellular expression. In contrast to HLA-G, the expression and function of HLA-E and HLA-F in physiologic and pathologic processes remain poorly established. In the present study, we show that HLA-E protein expression in normal human nonlymphoid organs is mainly restricted to endothelial cells (ECs). HLA-E is also basally expressed by B and T lymphocytes, natural killer (NK) cells and by macrophages. We demonstrate that tumor necrosis factor (TNF), interleukin-1ß (IL-1ß), and interferon (IFN) up-regulate the cell-surface expression of HLA-E on ECs in vitro and induce the release of soluble HLA-E (sHLA-E). HLA-E up-regulation protects IFN-activated ECs from NK-mediated cell lysis, while sHLA-E protects bystander cells. Finally, sHLA-E is not detected in normal sera, and increased serum levels correlate with disease activity in patients with antineutrophil cytoplasmic antibody–associated systemic vasculitis. Thus, HLA-E expression and release of sHLA-E are features of EC activation and emphasize immunoregulatory functions of the endothelium. The present identification of soluble HLA-E molecules may have important implications in understanding the pathogenesis of immune-mediated vascular diseases and for the diagnosis and monitoring of patients. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Iannello, O. Debbeche, S. Samarani, and A. Ahmad Antiviral NK cell responses in HIV infection: I. NK cell receptor genes as determinants of HIV resistance and progression to AIDS J. Leukoc. Biol., July 1, 2008; 84(1): 1 - 26. [Abstract] [Full Text] [PDF]

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