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Blood, 1 April 2007, Vol. 109, No. 7, pp. 2863-2870. Prepublished online as a Blood First Edition Paper on December 5, 2006; DOI 10.1182/blood-2006-06-027060. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-06-027060v1 109/7/2863    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Monteiro, M. Articles by Rocha, B. Search for Related Content PubMed PubMed Citation Articles by Monteiro, M. Articles by Rocha, B. Related Collections Immunobiology Gene Expression Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Cartography of gene expression in CD8 single cells: novel CCR7– subsets suggest differentiation independent of CD45RA expression Marta Monteiro1, César Evaristo1, Agnès Legrand1, Antonino Nicoletti2, and Benedita Rocha1 1 Institut National de la Santé et de la Recherche Médicale (INSERM) Unit 591, Necker Institute, René-Descartes Medical School, Paris, France; 2 INSERM Unit 681, Cordeliers Institute, Paris, France Understanding the distribution, function, and lineage relationship of CD8+ T-cell subpopulations is of fundamental value for the monitoring of the immune system in several experimental and clinical situations. However, the available data concerning the description of effector and memory CD8+ subsets in humans remain rather fragmentary because different studies favored the usage of distinct and restricted sets of cell surface markers and functional parameters. We associated multiple markers to subdivide CD8+ T cells into 14 different cell types, several of which were not described previously, and evaluated the coexpression of 18 genes simultaneously in individual cells from each subset. Our results show that each subset has a defined pattern of gene expression. Moreover, effector gene expression of CCR7– cells correlated only with CD27 expression levels and CD27/CD28 coexpression but not with CD45RA/R0 phenotypes. Our findings thus describe new CD8+ cell subsets, allow the identification of relatively homogeneous CD8+ subpopulations, provide a predictable and precise correlation between particular cell surface markers and CD8+ T-cell functional properties, and identify effector cells present in both CCR7–CD45RA+ and CCR7–CD45R0+ compartments. The results also indicate that activated cells might modulate the expression of CD45RA/R0 asynchronously rather than CCR7–CD45RA+ cells always issuing from CD45RA– precursors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I. Munitic, H. Decaluwe, C. Evaristo, S. Lemos, M. Wlodarczyk, A. Worth, A. Le Bon, L. K. Selin, Y. Riviere, J. P. Di Santo, et al. Epitope Specificity and Relative Clonal Abundance Do Not Affect CD8 Differentiation Patterns during Lymphocytic Choriomeningitis Virus Infection J. Virol., November 15, 2009; 83(22): 11795 - 11807. [Abstract] [Full Text] [PDF] V. Libri, D. Schulte, A. van Stijn, J. Ragimbeau, L. Rogge, and S. Pellegrini Jakmip1 Is Expressed upon T Cell Differentiation and Has an Inhibitory Function in Cytotoxic T Lymphocytes J. Immunol., November 1, 2008; 181(9): 5847 - 5856. [Abstract] [Full Text] [PDF] A. Peixoto, C. Evaristo, I. Munitic, M. Monteiro, A. Charbit, B. Rocha, and H. Veiga-Fernandes CD8 single-cell gene coexpression reveals three different effector types present at distinct phases of the immune response J. Exp. Med., May 14, 2007; 204(5): 1193 - 1205. [Abstract] [Full Text] [PDF]

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