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Blood, 1 April 2007, Vol. 109, No. 7, pp. 3099-3107. Prepublished online as a Blood First Edition Paper on November 21, 2006; DOI 10.1182/blood-2006-08-040139. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-08-040139v1 109/7/3099    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schirmer, D. A. Articles by Spitalnik, S. L. Search for Related Content PubMed PubMed Citation Articles by Schirmer, D. A. Articles by Spitalnik, S. L. Related Collections Transfusion Medicine Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSFUSION MEDICINE Mouse models of IgG- and IgM-mediated hemolysis David A. Schirmer1, Shuh-Chyung Song1, Jeffrey P. Baliff2, Stephanie O. Harbers3, Raphael A. Clynes3, Anna Krop-Watorek4, Gregory R. Halverson5, Marcin Czerwinski4, and Steven L. Spitalnik1 1 Department of Pathology, Columbia University, New York, NY; 2 Department of Pathology and Laboratory Medicine, University of Rochester, NY; 3 Department of Microbiology, Columbia University, New York, NY; 4 Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland; 5 Department of Immunochemistry, New York Blood Center, New York, NY Well-characterized mouse models of alloimmune antibody-mediated hemolysis would provide a valuable approach for gaining greater insight into the pathophysiology of hemolytic transfusion reactions. To this end, mouse red blood cells (mRBCs) from human glycophorin A transgenic (hGPA-Tg) donor mice were transfused into non-Tg recipients that had been passively immunized with IgG or IgM hGPA-specific monoclonal antibodies (mAbs). In this novel murine "blood group system," mRBCs from hGPA-Tg mice are "antigen positive" and mRBCs from non-Tg mice are "antigen negative." Passive immunization of non-Tg mice with the IgG1 10F7 and IgG3 NaM10-2H12 anti-hGPA mAbs each induced rapid clearance of incompatible transfused hGPA-Tg-mRBCs in a dose-response manner. Using various knockout mice as transfusion recipients, both the complement system and activating Fc receptors were found to be important in the clearance of incompatible mRBCs by each of these IgG mAbs. In addition, the IgM E4 anti-hGPA mAb induced complement-dependent intravascular hemolysis of transfused incompatible hGPA-Tg-mRBCs accompanied by gross hemoglobinuria. These initial studies validate the relevance of these new mouse models for addressing important questions in the field of transfusion medicine. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. A. Hod, C. M. Cadwell, J. S. Liepkalns, J. C. Zimring, S. A. Sokol, D. A. Schirmer, J. Jhang, and S. L. Spitalnik Cytokine storm in a mouse model of IgG-mediated hemolytic transfusion reactions Blood, August 1, 2008; 112(3): 891 - 894. [Abstract] [Full Text] [PDF] J. C. Zimring, C. M. Cadwell, T. E. Chadwick, S. L. Spitalnik, D. A. Schirmer, T. Wu, C. A. Parkos, and C. D. Hillyer Nonhemolytic antigen loss from red blood cells requires cooperative binding of multiple antibodies recognizing different epitopes Blood, September 15, 2007; 110(6): 2201 - 2208. [Abstract] [Full Text] [PDF]

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