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Blood, 15 April 2007, Vol. 109, No. 8, pp. 3260-3269. Prepublished online as a Blood First Edition Paper on December 27, 2006; DOI 10.1182/blood-2006-07-036269. This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: blood-2006-07-036269v1 109/8/3260    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Macaulay, I. C. Articles by Ouwehand, W. H. Search for Related Content PubMed PubMed Citation Articles by Macaulay, I. C. Articles by Ouwehand, W. H. Related Collections Hematopoiesis and Stem Cells Gene Expression Genomics Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins Iain C. Macaulay1,2, Marloes R. Tijssen3,4, Daphne C. Thijssen-Timmer3,4, Arief Gusnanto5, Michael Steward6, Philippa Burns1,2, Cordelia F. Langford7, Peter D. Ellis7, Frank Dudbridge5, Jaap-Jan Zwaginga3,4,8, Nicholas A. Watkins1,2, C. Ellen van der Schoot3,4,9, and Willem H. Ouwehand1,2 1 Department of Haematology, University of Cambridge, United Kingdom; 2 National Blood Service, Cambridge, United Kingdom; 3 Department of Experimental Immunohaematology, Sanquin Research at Central Laboratory for the Blood Transfusion Service (CLB), Amsterdam, The Netherlands; 4 Landsteiner Laboratory, Academic Medical Center (AMC), University of Amsterdam, The Netherlands; 5 Medical Research Council (MRC) Biostatistics Unit, Institute of Public Health, Cambridge, United Kingdom; 6 Domantis Limited, Cambridge, United Kingdom; 7 Microarray Facility, Wellcome Trust Sanger Institute, Cambridge, United Kingdom; 8 Department of Immunohematology–Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands; 9 Department of Hematology, Amsterdam Medical Centre, University of Amsterdam, The Netherlands To identify previously unknown platelet receptors we compared the transcriptomes of in vitro differentiated megakaryocytes (MKs) and erythroblasts (EBs). RNA was obtained from purified, biologically paired MK and EB cultures and compared using cDNA microarrays. Bioinformatical analysis of MK–up-regulated genes identified 151 transcripts encoding transmembrane domain-containing proteins. Although many of these were known platelet genes, a number of previously unidentified or poorly characterized transcripts were also detected. Many of these transcripts, including G6b, G6f, LRRC32, LAT2, and the G protein–coupled receptor SUCNR1, encode proteins with structural features or functions that suggest they may be involved in the modulation of platelet function. Immunoblotting on platelets confirmed the presence of the encoded proteins, and flow cytometric analysis confirmed the expression of G6b, G6f, and LRRC32 on the surface of platelets. Through comparative analysis of expression in platelets and other blood cells we demonstrated that G6b, G6f, and LRRC32 are restricted to the platelet lineage, whereas LAT2 and SUCNR1 were also detected in other blood cells. The identification of the succinate receptor SUCNR1 in platelets is of particular interest, because physiologically relevant concentrations of succinate were shown to potentiate the effect of low doses of a variety of platelet agonists. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: MEGAprofiles provide big insightsinto platelet function Wadie F. Bahou Blood 2007 109: 3129-3130. [Full Text] [PDF] This article has been cited by other articles: D. Q. Tran, J. Andersson, R. Wang, H. Ramsey, D. Unutmaz, and E. M. Shevach GARP (LRRC32) is essential for the surface expression of latent TGF-{beta} on platelets and activated FOXP3+ regulatory T cells PNAS, August 11, 2009; 106(32): 13445 - 13450. [Abstract] [Full Text] [PDF] A. S. Weyrich and G. A. Zimmerman Comparative genomics: fishing nets hemostatic catch Blood, May 7, 2009; 113(19): 4479 - 4480. [Full Text] [PDF] N. A. Watkins, A. Gusnanto, B. de Bono, S. De, D. Miranda-Saavedra, D. L. Hardie, W. G. J. Angenent, A. P. Attwood, P. D. Ellis, W. Erber, et al. A HaemAtlas: characterizing gene expression in differentiated human blood cells Blood, May 7, 2009; 113(19): e1 - e9. [Abstract] [Full Text] [PDF] M. N. O'Connor, I. I. Salles, A. Cvejic, N. A. Watkins, A. Walker, S. F. Garner, C. I. Jones, I. C. Macaulay, M. Steward, J.-J. Zwaginga, et al. Functional genomics in zebrafish permits rapid characterization of novel platelet membrane proteins Blood, May 7, 2009; 113(19): 4754 - 4762. [Abstract] [Full Text] [PDF] N. Soranzo, A. Rendon, C. Gieger, C. I. Jones, N. A. Watkins, S. Menzel, A. Doring, J. Stephens, H. Prokisch, W. Erber, et al. A novel variant on chromosome 7q22.3 associated with mean platelet volume, counts, and function Blood, April 16, 2009; 113(16): 3831 - 3837. [Abstract] [Full Text] [PDF] F. Feroze-Zaidi, L. Fusi, M. Takano, J. Higham, M. S. Salker, T. Goto, S. Edassery, K. Klingel, K. M. Boini, M. Palmada, et al. Role and Regulation of the Serum- and Glucocorticoid-Regulated Kinase 1 in Fertile and Infertile Human Endometrium Endocrinology, October 1, 2007; 148(10): 5020 - 5029. [Abstract] [Full Text] [PDF] S. A. Newland, I. C. Macaulay, A. R. Floto, E. C. de Vet, W. H. Ouwehand, N. A. Watkins, P. A. Lyons, and D. R. Campbell The novel inhibitory receptor G6B is expressed on the surface of platelets and attenuates platelet function in vitro Blood, June 1, 2007; 109(11): 4806 - 4809. [Abstract] [Full Text] [PDF]

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