Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 15 April 2007, Vol. 109, No. 8, pp. 3505-3508.
Prepublished online as a Blood First Edition Paper on December 19, 2006; DOI 10.1182/blood-2006-08-043570.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Methods, Table, Figure, and Video
Right arrow All Versions of this Article:
blood-2006-08-043570v1
109/8/3505    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lam, W. A.
Right arrow Articles by Fletcher, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lam, W. A.
Right arrow Articles by Fletcher, D. A.
Related Collections
Right arrow Hemostasis, Thrombosis, and Vascular Biology
Right arrow Cytoskeleton
Right arrow Brief Reports
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

NEOPLASIA

Brief Report

Chemotherapy exposure increases leukemia cell stiffness

Wilbur A. Lam1,2, Michael J. Rosenbluth2, and Daniel A. Fletcher2,3

1 Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of California, San Francisco (UCSF); 2 Department of Bioengineering and UCSF/University of California, Berkeley, (UCB) Joint Graduate Group in Bioengineering, University of California, Berkeley; 3 Biophysics Graduate Group, University of California, Berkeley

Deformability of blood cells is known to influence vascular flow and contribute to vascular complications. Medications for hematologic diseases have the potential to modulate these complications if they alter blood cell deformability. Here we report the effect of chemotherapy on leukemia cell mechanical properties. Acute lymphoblastic and acute myeloid leukemia cells were incubated with standard induction chemotherapy, and individual cell stiffness was tracked with atomic force microscopy. When exposed to dexamethasone or daunorubicin, leukemia cell stiffness increased by nearly 2 orders of magnitude, which decreased their passage through microfluidic channels. This stiffness increase occurred before caspase activation and peaked after completion of cell death, and the rate of stiffness increase depended on chemotherapy type. Stiffening with cell death occurred for all cell types investigated and may be due to dynamic changes in the actin cytoskeleton. These observations suggest that chemotherapy itself may increase the risk of vascular complications in acute leukemia.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Lautenschlager, S. Paschke, S. Schinkinger, A. Bruel, M. Beil, and J. Guck
The regulatory role of cell mechanics for migration of differentiating myeloid cells
PNAS, September 15, 2009; 106(37): 15696 - 15701.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2007 by American Society of Hematology         Online ISSN: 1528-0020