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Blood, 15 April 2007, Vol. 109, No. 8, pp. 3544-3551. Prepublished online as a Blood First Edition Paper on December 7, 2006; DOI 10.1182/blood-2006-07-035139. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2006-07-035139v1 109/8/3544    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by El Nemer, W. Articles by Le Van Kim, C. Search for Related Content PubMed PubMed Citation Articles by El Nemer, W. Articles by Le Van Kim, C. Related Collections Cell Adhesion and Motility Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  RED CELLS Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell 4ß1integrin: role in adhesion of sickle red blood cells to endothelial cells Wassim El Nemer1,3, Marie-Paule Wautier1,3, Cécile Rahuel1,3, Pierre Gane1,3, Patricia Hermand1,3, Frédéric Galactéros4, Jean-Luc Wautier2,3, Jean-Pierre Cartron1,3, Yves Colin1,3, and Caroline Le Van Kim1,3 1 Institut National de la Santé et de la Recherche Médicale (INSERM) U665, Paris, France; 2 Institut National de la Transfusion Sanguine, Paris, France; 3 University Paris 7, France; 4 Hôpital Henri Mondor, Assistance Publique Hôpitaux Paris (APHP), Créteil, France The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin 5 chain. In addition to red blood cells, Lu/BCAM proteins are highly expressed in endothelial cells. Abnormal adhesion of red blood cells to the endothelium could potentially contribute to the vaso-occlusive episodes in sickle cell disease. Considering the presence of integrin consensus-binding sites in Lu/BCAM proteins, we investigated their potential interaction with integrin 4ß1, the unique integrin expressed on immature circulating sickle red cells. Using cell adhesion assays under static and flow conditions, we demonstrated that integrin 4ß1 expressed on transfected cells bound to chimeric Lu-Fc protein. We showed that epinephrine-stimulated sickle cells, but not control red cells, adhered to Lu-Fc via integrin 4ß1 under flow conditions. Antibody-mediated activation of integrin 4ß1 induced adhesion of sickle red cells to primary human umbilical vein endothelial cells; this adhesion was inhibited by soluble Lu-Fc and vascular cell adhesion molecule-1 (VCAM-1)–Fc proteins. This novel interaction between integrin 4ß1 in sickle red cells and endothelial Lu/BCAM proteins could participate in sickle cell adhesion to endothelium and potentially play a role in vaso-occlusive episodes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M.-H. Odievre, V. Bony, M. Benkerrou, C. Lapoumeroulie, C. Alberti, R. Ducrocq, E. Jacqz-Aigrain, J. Elion, and J.-P. Cartron Modulation of erythroid adhesion receptor expression by hydroxyurea in children with sickle cell disease Haematologica, April 1, 2008; 93(4): 502 - 510. [Abstract] [Full Text] [PDF] W. R. Smith, L. T. Penberthy, V. E. Bovbjerg, D. K. McClish, J. D. Roberts, B. Dahman, I. P. Aisiku, J. L. Levenson, and S. D. Roseff Daily Assessment of Pain in Adults with Sickle Cell Disease Ann Intern Med, January 15, 2008; 148(2): 94 - 101. [Abstract] [Full Text] [PDF]

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