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Blood, 1 May 2007, Vol. 109, No. 9, pp. 3922-3928. Prepublished online as a Blood First Edition Paper on January 30, 2007; DOI 10.1182/blood-2006-09-046391. This Article Full Text Full Text (PDF) Supplemental Table All Versions of this Article: blood-2006-09-046391v1 blood-2006-09-046391v2 109/9/3922    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sakhinia, E. Articles by Byers, R. J. Search for Related Content PubMed PubMed Citation Articles by Sakhinia, E. Articles by Byers, R. J. Related Collections Gene Expression Genomics Clinical Trials and Observations Neoplasia Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA Clinical quantitation of diagnostic and predictive gene expression levels in follicular and diffuse large B-cell lymphoma by RT-PCR gene expression profiling Ebrahim Sakhinia1, Caroline Glennie1, Judith A. Hoyland1, Lia P. Menasce2, Gerard Brady3, Crispin Miller4, John A. Radford5, and Richard J. Byers1 1 Division of Regenerative Medicine, School of Medicine, Faculty of Medical and Human Sciences, The University of Manchester, Manchester, United Kingdom; 2 Department of Histopathology, Christie Hospital National Health Service (NHS) Trust, Manchester, United Kingdom; 3 Epistem Ltd, Manchester, United Kingdom; 4 Paterson Institute for Cancer Research, Manchester, United Kingdom; 5 Cancer Research United Kingdom Department of Medical Oncology, The University of Manchester and Christie Hospital NHS Trust, Manchester, United Kingdom Recent microarray gene expression profiling studies have identified gene signatures predictive of outcome, so-called "indicator" genes, for diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL). However, measurement of these genes in routine practice remains difficult. We applied real-time polymerase chain reaction (PCR) to polyA cDNAs prepared from 106 archived human frozen lymph nodes (63 of FL, 25 of DLBCL, 10 reactive lymph nodes, and cases with paired samples of FL [4] and subsequent DLBCL [4]). Reverse transcription and polyA reverse transcriptase (RT)–PCR was performed, and resultant cDNA was probed by real-time PCR for 36 candidate indicator genes, selected from microarray studies. Nine genes showed statistically significant different expression between FL and DLBCL, including cyclin B, COL3A1, NPM3, H731, PRKCB1, OVGL, ZFPC150, HLA-DQ-a, and XPB. Of these, cyclin B, NPM3, and COL3A1 were higher in DLBCL. Six genes showed statistically significant higher expression in the neoplastic nodes compared with reactive nodes, namely PRKCB1, BCL-6, EAR2, ZFX, cyclin B, YY1. High levels of YY.1 were associated with a shorter survival interval in both FL and DLBCL. The method is simple, sensitive, and robust, facilitating routine use and may be used as a platform for clinical measurement of prognostic gene signatures. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Gene expression levels in lymphoma Philip S. Rosenberg Blood 2007 109: 3621. [Full Text] [PDF] This article has been cited by other articles: R. J. Byers, E. Sakhinia, P. Joseph, C. Glennie, J. A. Hoyland, L. P. Menasce, J. A. Radford, and T. Illidge Clinical quantitation of immune signature in follicular lymphoma by RT-PCR-based gene expression profiling Blood, May 1, 2008; 111(9): 4764 - 4770. [Abstract] [Full Text] [PDF]

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